Com this study was carried out to isolate the active

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: sults, dichloromethane and n-butanol fractions showed the higher antimicrobial activity than other solvents. Purificat ion of nbutanol fraction was done by using Sephadex LH-20, normal phase column chromatography (eluted with n-butanol: acetic acid: water (12:3:5)) and Reverse Phase column chromatography (eluted with water/acetonitrile and acetonitrile/methanol) to yield four compounds (1-4). All compounds were tested for their antibacterial activityagainst Bacillus anthracis. The results showed that both compound 3 and 4 exhibited a significant antibacterial activity. Although the spectroscopy (NMR and FTIR) analysis was performed, research is still in progress to elucidate the structures of the isolated compounds. NAT-P15 KAEMPFEROL AND PINORESINOL FROM CUSCUTA CAMPESTRIS YUNCKER 1 Leong Sow Tein, Khalijah Awang, Muhammad Remy Othman and Baki Bakar 1 2 2 2 Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia E-mail: [email protected] 1 Cuscuta campestris Yuncker (field dodder), a member of the Convolvulaceae family, is an annual obligate stem parasite that twined on other plants and attaches to the above -ground parts of a wide range of host plants. This plant can be found in abandoned area like shrubs and bushes along the roadside. Cuscuta campestris was collected from Johor, Malaysia and dried. 200 g of dried Cuscuta campestris was extracted with ethanol at room temperature and dried with freeze dryer. Separation and purification of chemical compounds were done by using chrom atography method. Identification of chemical compounds in Cuscuta campestris was carried out by TLC, LC-MS and NMR. The structures of kaempferol and pinoresinol were established by the NMR spectral and MS spectral by comparison of these data to the literature value. Kaempferol Pinoresinol 178 3rd ICYC 2010 Universiti Sains Malaysia NAT-P16 23rd-25th June 2010 INDOLE ALKALOIDS ISOLATED FROM NEONAUCLEA SP-1 (RUBIA...
View Full Document

This note was uploaded on 02/17/2011 for the course CHEMISTRY 101 taught by Professor Csr during the Spring '11 term at University of Louisville.

Ask a homework question - tutors are online