Edumy 2 chloropyrimidine which was obtained

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Unformatted text preview: ctroscopic analysis. Fluorescence studies of the ligands and their copper(II) complexes were carried out using methanol as the solvent. In general, metal ions are able to quench the fluorescence of organic ligands. The complexes each exh ibit characteristics fluorescence, with 2-N-ethylaminopyrimidine showed a high relative intensity compared to 2-N-anilinopyrimidine complex. 131 23rd-25th June 2010 3rd ICYC 2010 Universiti Sains Malaysia INO-O18 ENCAPSULATION OF ALKALINE PHOSPHATASE IN MESOPOROUS METHYL-SILICA HYBRID BY SOL-GEL PROCESS 1 Sriyanti, Eko Sri Kunarti and Narsito 2 2 Department of Chemistry, Faculty of Mathematics and Natural Science, Diponegoro University, Semarang, Indonesia 50275 2 Department of Chemistry, Faculty of Mathematics and Natural Science, Gadjah Mada University, Yogyakarta, Indonesia E-mail: In recent years, the sol-gel technique has attracted increasing interest as a unique approach to immobilize biomolecules for bioanalitical applications as well as biochemical and biophysical studies. In this research, encapsulation of Alkaline Phosphatase (ALP) enzyme in mesoporous methyl -silica hybrid by sol-gel process has been carried out. Mesoporous methyl -silica hybrid has been synthesis by using tetraethylorthosilicate (TEOS) and methyltriethoxysilane (MTES) as precursor and poly(ethylene) glycol (PEG) as a polymer dopant. The preparation of methyl -silica hybrid was carried out at various mol ratios of precursors and PEG concentration. Encapsulation was emphasized by mixing sol solution and tris -HCl buffer containing ALP. The Surface Area Analyzer analysis data showed that the pore diameter and surface area of methyl-silica hybrid tended to increase as the PEG dopant concentration increases. Result showed that ALP encapsulated in the methyl-silica hybrids with PEG dopant gave enzymatic activity as 0.112 U/mg enzyme, higher than ALP encapsulated in copolymerization of TEOS with MTES or in silica from TEOS precursor only (0.098 U/mg enzyme and 0.069 U/mg enzyme, respectively). It was shown that copolymerizatio n of TEOS with organosilane precursor and the entrapm...
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