Unformatted text preview: ano -materials can provide a larger surface area for biomolecule attachment apart from improving conductivity in the case of metallic nanoparticles when applied to electrochemical biosensors. These properties of nano-materials resulted in good analyte diffusion and easy signal transduction to the electrode and hence improve the overall analytical performance of the biosensor such as response time, linear response range a nd detection limit. In this paper we discuss the use of a new nano-material such as acrylic polymer nanospheres for biosensor fabrication. These hydrophobic acrylic materials can be prepared using rapid and direct photopolymerisation of acrylate monomers in aqueous phase. These acrylic spheres of size from 200 500 nm with surface modification allowed the attachment of biomolecules such as enzymes and DNA probes. Because of the good adhesion nature of these micro/nanospheres, they can be coated directly onto the electrode surface, without the loss of the bio-recognition elements of the biosensor. The improved performance of the biosensors for urea, formaldehyde and DNA based on micro/nanospheres and gold nano-particles using potentiometric, amperometric and optical transductions will be discussed. KN-18 CHIRALLY FUNCTIONALIZED STATIONARY PHASE FOR ENANTIOMERIC SEPARATION OF AMINO ACIDS IN CAPILLARY LC Lee Wah Lim and Toyohide Takeuchi Department of Chemistry, Faculty of Engineering, Gifu University, 1 -1 Yanagido, Gifu 501-1193, Japan E-mail: [email protected] The separation of chiral compounds has been of great interest because the majority of biomolecules such as amino acids, sugars, proteins, nucleic acids, etc, are chiral. In nature, some of these biomolecules (except macromolecules) exist either in the L-form or the D-form, which are normally called enantiomers or optical isomers, and the separation of these enantiomers is very important because some enantiomers show totally different biological activities than their optical isomers. In liquid chromatography (LC), enantiomer...
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This note was uploaded on 02/17/2011 for the course CHEMISTRY 101 taught by Professor Csr during the Spring '11 term at University of Louisville.
- Spring '11