Health20100100009_59638008 - Vol.2, No.1, 51-61 (2010)

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Vol.2, No.1, 51-61 (2010) doi:10.4236/health.2010.21009 SciRes Copyright © 2010 Health Openly accessible at Bioengineering functional copolymers. XII. Interaction of boron-containing and PEO branched derivatives of poly(MA- alt -MVE) with HeLa cells Mustafa Türk 1 , Zakir M. O. Rzayev 2,* , Gülcihan Kurucu 2 1 Department of Biology, Faculty of Science and Art, K ı r ı kkale University, Yahsihan, 71450 Kirikkale, Turkey 2 Department of Chemical Engineering, Faculty Engineering, Hacettepe University, Beytepe, 06800 Ankara, Turkey; Correspondence author: Received 20 October 2009; revised 20 November 2009; accepted 11 November 2009. ABSTRACT Novel boron-containing bioengineering copoly- mer and its α -hydoxy- ω -methoxy-poly(ethy- lene oxide (PEO) macrobranched derivatives were synthesized by (1) partially amidolysis of poly(maleic anhydride- alt -methyl vinyl ether) w ı th ethanolamine ester of diphenylboronic acid and (2) esterification of synthesized B-con- taining copolymers with PEO. They had a com- bination of hydrophilic/hydrophobic linkages, free carboxylic groups, positive charges and an ionized organoboron linkage as antitumor sites, along with an ability to interact with HeLa cells. The structure, composition and properties (cy- totoxicity and antitumor activity) of synthe- sized copolymers were investigated. In vitro cytotoxicity results, obtained by the fluore scence microscopy measurements indicate that unlike the virgin copolymer, boron-containing and PEO macrobranched derivatives exhibit higher antitumor activity. It was found that organoboron copolymer exhibits the most apo- ptotic and necrotic effects against HeLa cells whereas a minor effect relative to cancer cells was observed on L929 Fibroblast cells. Keywords: Synthesis; Organoboron Copolymers; Structure-Property Relations; Cytotoxicity; Antitumor Activity; Apoptotic Effect 1. INTRODUCTION The bioengineering functional polymers exhibit the characteristics of 1) alternating and random copolymers of maleic anhydride (MA) and 2) poly(ethylene oxide) (PEO), as well as 3) PEO grafted functional macro- molecules. They are of great interest for many researchers due to their nontoxic, cell-compatible, biodegradable, stimuli-responsive properties, and therefore, a wide range of biomedical and bioengineering applications exist as drug or enzyme carriers and biomacromolecular conju- gates both in diagnostics and chemotherapy as effective antitumor agents [1-8]. It is known that these copolymers can be regarded as pre-activated polymers due to the presence of anhydride moieties susceptible to the reaction with a primary amine of a biomolecule [9]. The alternat- ing copolymers of maleic anhydride (MA) with methyl vinyl ether (MVE) or divinyl ether (DVE) were utilized in various applications in diagnostics [10,11] and in che- motherapy as effective antitumor agents [8]. Poly (MA- alt -DVE), known as pyran copolymer is one of the well known bioengineering polymers having a wide
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This note was uploaded on 02/17/2011 for the course CHEMISTRY 101 taught by Professor Csr during the Spring '11 term at University of Louisville.

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Health20100100009_59638008 - Vol.2, No.1, 51-61 (2010)

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