T000675 - IDENTIFICATION OF GENES THAT PLAY ROLES IN BORON...

Info iconThis preview shows pages 1–5. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: IDENTIFICATION OF GENES THAT PLAY ROLES IN BORON METABOLISM A Thesis Submitted to the Graduate School of Engineering and Sciences of İ zmir Institute of Technology in Partial Fulfillment of the Requirements for the Degree of MASTER OF SCIENCE in Biotechnology by Alaattin KAYA July 2008 İ ZM İ R We approve the thesis of Alaattin KAYA ______________________________ Assist. Prof. Dr. Ahmet KOÇ Supervisor ______________________________ Prof. Dr.Ahmet ERO Ğ LU Co-Supervisor ______________________________ Assoc. Prof. Dr. Anne FRARY Committee Member ______________________________ Assoc. Prof. Dr. Talat YALÇIN Committee Member ______________________________ Assist. Prof. Dr. H. Ça ğ lar KARAKAYA Committee Member ______________________________ Assist. Prof. Dr. Alper ARSLANO Ğ LU Committee Member 10 July 2008 ________________ Date _____________________________ _______________________ Prof. Dr. Semra ÜLKÜ Prof. Dr. Hasan BÖKE Head of the Biotechnology Department Dean of the Graduate School of Engineering and Sciences ACKNOWLEDGMENTS First of all, I would like to thank my supervisor Assist. Prof. Dr. Ahmet KOÇ who give me the chance to study in this delightful project, his guidance, beneficial suggestions, his support and help not only directly related with the project, but also with all the troubles I have met, and for always being there for me whenever I needed him. I would like to thank Assist. Prof. Dr. Ça ğ lar KARAKAYA for his guidance and moral support during my studies and also his trustworthy friendship. I also would like to thank Assoc. Prof. Dr. Talat YALÇIN, Assist. Prof. Dr. Alper ARSLANOGLU and Assoc. Prof. Dr. Anne FRARY for their guidance and help. I would like to thank my friends and lab mates Ercan Selçuk ÜNLÜ, Ersin AKINCI, Ahmet Emin AT İ K, Elise HACIO Ğ LU, Hasan Cihad TEKEDAR and Erhan BAL for their sincere help and their good friendship. I also wish to express my thanks to all my other friends working in the Molecular Genetic Laboratory. Finally, I would like to thank my family for both the financial and moral support they showed in every stage of my life. They will always be in my heart. I also would like to thank The Scientific and Technological Research Council of Turkey (TUBITAK) for their financial support, it was important for my success to have TUBITAK’s scholarship. iv ABSTRACT IDENTIFICATION OF GENES THAT PLAY ROLES IN BORON METABOLISM Boron, is an essential microelement that plays a role in plant and animal development. However, an excess amount of boron is toxic for both types of organisms. The yeast Saccharomyces cerevisiae is an ideal model organism for describing the mechanism and regulation of metal ion transport in eukaryotes. We screened a yeast genomic DNA library to find genes that confer boron resistance to wild-type cells.Thirty transformants were isolated that were able to grow in the presence of a toxic amount of boron and all of them contained the multidrug efflux transporter gene ATR1 (YML116w)...
View Full Document

This note was uploaded on 02/17/2011 for the course CHEMISTRY 101 taught by Professor Csr during the Spring '11 term at University of Louisville.

Page1 / 42

T000675 - IDENTIFICATION OF GENES THAT PLAY ROLES IN BORON...

This preview shows document pages 1 - 5. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online