101A_F10_Final_key

101A_F10_Final_key - MCDB 101A Fall 10 Final 12/10/2010...

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1 MCDB 101A Fall 10 Final Name____ Key__________________ _________________ 12/10/2010 (207 points total) Perm #__________________________________________ Multiple Choice: Circle the letter corresponding to the best answer (3 points for each correct answer) 1. In wobble base-pairing a. the number of codons needed to specify all 20 amino acids is less than 20 b. the 61 sense codons can be read by fewer than 61 different tRNAs c. some bases in the 3’ position of the anticodon sequence may pair with multiple mRNA bases d. a single termination/release factor will recognize all three stop codons e. both b and c are correct 2. If Meselson and Stahl only examined E. coli DNA from the first round of replication following the transfer of the bacteria to 14 N media, they would have concluded that a. DNA replication is semiconservative b. DNA replication is conservative c. DNA replication is either semiconservative or dispersive d. DNA replication is either conservative or dispersive e. neither of the three models could be ruled out 3. Which of the following is an example of a conditional mutation? a. A mutation that makes an organism heat sensitive b. A host range phage mutation c. A mutation that makes an organism light sensitive d. A mutation that blocks the synthesis of an essential metabolite e a, b, or c 4. A double stranded DNA molecule consists of 35% guanine. The adenine content of the molecule a. is 15% b. is 20% c. is 25% d. is 35% e. cannot be determined from the information given. 5 . Which of the following is a correct statement about the Shine Delgarno sequence? a. This is the sequence of DNA that encodes the Rho-binding site b. This is the sequence of rRNA that encodes the mRNA binding site c. This is the sequence of DNA that encodes the Sigma factor binding site d. This is the sequence of mRNA that binds to the 50s ribosomal subunit e. None of these statements are correct 6. DNA phosphatase is used in cloning experiments for the purpose of a. preventing self-ligation of vectors cut with a single restriction enzyme b. enhancing the activity of certain restriction endonucleases c. reducing the time required for ligation of the gene insert with the cut vector d. enhancing the activity of DNA ligase e. digesting unwanted DNA molecules
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2 7. The addition of 5 nucleotides between the -35 and -10 regions of a bacterial promoter would a. increase binding of the RNA polymerase holoenzyme b. increase binding of the RNA polymerase core complex c. decrease binding of the RNA polymerase holoenzyme d. have no effect on the binding of either form of RNA polymerase e. increase the binding of Rho protein 8. A defect in the epsilon subunits of DNA polymerase III would most likely lead to a deficiency in its a. polymerase activity b. processivity c. primase activity d. 5’-3’ endonuclease activity e. 3’-5’ exonuclease activity 9. The process by which a specialized transducing phage is formed is most similar to a. non-replicative transposition b. rho-independent transcriptional termination
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101A_F10_Final_key - MCDB 101A Fall 10 Final 12/10/2010...

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