101A_F10_midterm1key

101A_F10_midterm1key - MCDB 101A Midterm 1 10/19/10 (100...

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1 MCDB 101A Midterm 1 Name____ Key__________________ _________________ 10/19/10 (100 points total) Perm #__________________________________________ Multiple Choice: Circle the letter corresponding to the best answer (3 points for each correct answer) 1. Which of the following is true if a DNA molecule consists of 20% A, 30% T and 20% G? a. The C content of the molecule must be 30% b. The C content of the molecule must be less than 30% c. The C content of the molecule cannot be determined from the information given d. The molecule is not double stranded e. Both a and d are correct 2. Which of the following is the correct order of steps to clone a gene? (GOI = gene of interest) a. Isolate GOI Æ Insert into Plasmid Æ Transform Æ Plasmid Isolation Æ Sequence b. Sequence Æ Isolate GOI Æ Insert into plasmid Æ Transform Æ Plasmid Isolation c. Isolate GOI Æ Insert into Plasmid Æ Plasmid Isolation Æ Transform Æ Sequence d. Transform Æ Isolate GOI Æ Insert into Plasmid Æ Plasmid Isolation Æ Sequence e. Plasmid Isolation Æ Isolate GOI Æ Insert into Plasmid Æ Sequence Æ Transform 3 . Which of the following is essential for the error-correcting abilities of DNA Pol I and III? a. Whether the DNA molecule it is a right-handed or left-handed helix b. The 3’-5’ exonuclease activity of the polymerase c. The antiparallel nature of the DNA molecule d. The 5’-3’ exonuclease activity of the polymerase e. Both b and c are correct 4. Which of the following information can be obtained from the trans test? a. The relative order of mutations on a chromosome b. The relative distance between mutations c. Whether mutations are in the same or different genes d. Whether mutations are dominant or recessive e. Both a and b 5. Which of the following is not a conditional mutation? a. A mutation that makes a cell more sensitive to cold than a wild type cell b. A mutation that blocks the transport of an essential metabolite into a cell c. A mutation that blocks the synthesis of an essential metabolite d. A bacteriophage host range mutation e. Neither b nor c are conditional mutations 6. You carry out a cis test between 2 bacteriophage having genotypes AbCd and aBcD. Assuming you have assays that allow you to distinguish all possible phenotypes associated with these markers how many different phenotypes would you expect to see? a. 4 b. 8 c. 16 d. 32 e. 64
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2 7. Allele “A” of phage X allows it to infect bacterial strains 1 and 2 whereas a phage with the “a” allele can only infect strain 1. The B allele yields slow lysis while a strain with the b allele exhibits rapid lysis. A mixture of strains 1 and 2 is infected with phage X. Large cloudy plaques are consistent with which genotype?
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101A_F10_midterm1key - MCDB 101A Midterm 1 10/19/10 (100...

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