M101A_2010Midterm1

M101A_2010Midterm1 - MCDB 101A Winter 2010 cover page Do...

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1 MCDB 101A Winter 2010 cover page Do not open until instructed to do so
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2 MCDB 101A W2010 Midterm 1 Name______ ______ _________________________ 1/25/10 (100 points total) Perm #____________________________________ Multiple Choice: Circle the letter corresponding to the best answer (3 points for each correct answer). For all questions concerning cis and trans tests involving phage, assume that wild type control strains were assayed in parallel with the mutants and that controls yielded plaques as expected. 1. A mutant organism that requires the amino acid tryptophan in order to grow is known as a/an a. prototroph b. auxotroph c. heterotroph d. merozygote e. conditional mutant 2. Franklin’s X-ray diffraction studies directly provided which of the following information? a. The total length of a DNA molecule b. The length of each turn of a DNA molecule c. The antiparallel structure of the DNA molecule d. All of the above e. both b and c 3. rII mutations of bacteriophage T4 are examples of a. auxotrophic mutations b. conditional mutations c. host range mutations d. temperature sensitive mutations e. both b and c 4. What will be the result if a dominant host range rII mutation is included in the trans test? a. complementation will be seen b. the two mutations will appear to be in different genes c. no plaques will be observed when the mutant strains coinfect a nonpermissive host d. plaques will be observed when the mutant strains coinfect a nonpermissive host e. both a and d 5. DNA phosphatase is used in cloning experiments for the purpose of a. preventing self-ligation of vectors cut with a single restriction enzyme b. enhancing the activity of certain restriction endonucleases c. reducing the time required for ligation of the gene insert with the cut vector d. lysing bacteria to obtain plasmid DNA e. cutting DNA molecules 6. When mapping bacteriophage deletions, mutant phage strains are added pairwise to a permissive bacterial strain and the cell lysate is assayed for plaque formation when added to a nonpermissive bacterial strain. The absence of plaques in the lysate + nonpermissive strain step indicates
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This note was uploaded on 02/18/2011 for the course MCDB 101A taught by Professor Thrower during the Spring '08 term at UCSB.

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M101A_2010Midterm1 - MCDB 101A Winter 2010 cover page Do...

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