M101A_W2010Midterm2KEY

M101A_W2010Midterm2K - MCDB 101A Midterm 2 Name_KEY 100 points total Perm Circle the correct choice for each of the following multiple choice

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1 MCDB 101A Midterm 2 Name______ KEY _____________________________ Feb 17, 2010 100 points total Perm#_______________________________________ Circle the correct choice for each of the following multiple choice questions (3 points each) 1. A mutation that changes the codon UUG to CUG is an example of a? a. Nonsense mutation b. Missense mutation c. Silent mutation d. Transition mutation e. Both c and d 2. A mutation within the Shine Delgarno sequence could result in which of the following? a. a truncated protein b. failure to initiate translation c. failure to initiate transcription d. failure to terminate transcription (for rho-dependent transcriptional termination) e. both b and d 3. Which protein is most similar in function to LacI? a. RecA b. CII c. CIII d. Cro e. N protein 4. Oxidative damage that generates 8-oxodG causes a. transition mutations b. transversion mutations c. both transition and transversion mutations d. deletions e. inversions 5. An “opal” suppressor (3’ACU 5’) is known to arise by mutation in a gene encoding a leu t-RNA. What would be the sequence of the template strand of the gene encoding the anticodon of this suppressor tRNA? a. 5’ TTA b. 5’ TCA c. 3’ UCA . d. 5’ AGU e. 5’ TGA 6. Shown below is a base pair that results from tautomerization. The bases are a. enol cytosine and keto adenine b. keto guanine and enol adenine c. imino thymine and enol cytosine d. amino cytosine and imino adenine e. pseudouracil and amino xanthine
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2 7. The presence of nucleotide repeats or exposure of DNA to intercalating agents is likely to generate a. apurinic sites b. nucleotide insertions/deletions c. frameshift mutations d. inversions e. both b and c 8. Genes A and B have mutation frequencies, respectively, of 1 X 10 -7 and 5 X 10 -8 mutations per cell per generation. With what frequency would you expect to find a bacterium that has mutations in both genes? a. 1.5 X 10 -7 b. 6 X 10 -7 c. 5 X 10 -15 d. 6 X 10 -15 e. 5 X 10 -56 9. Which correctly describes the blue-white cloning system? a. Blue colonies indicate successful integration of your gene into the cloning plasmid b. A multi-cloning site is located upstream of the lacZ gene contained in the cloning plasmid c. The N-terminal portion of the lacZ gene is present in the cloning plasmid d. A dominant mutation is present in the plasmid-encoded copy of lacZ e. both b and d 10. Which of the following modifications would be most likely to increase the specificity of the interaction between the labeled probe and target DNA in a Southern blot? a. Use more labeled probe DNA in the hybridization reaction
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This note was uploaded on 02/18/2011 for the course MCDB 101A taught by Professor Thrower during the Spring '08 term at UCSB.

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M101A_W2010Midterm2K - MCDB 101A Midterm 2 Name_KEY 100 points total Perm Circle the correct choice for each of the following multiple choice

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