M101A_Winter2010Final

M101A_Winter2010Final - MCDB 101A Final Exam March 17, 2010...

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1 MCDB 101A Final Exam Name_______________________________________ March 17, 2010 200 points total Perm#_______________________________________ MCDB 101A Winter 2010 cover page Do not open until instructed to do so
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2 Circle the correct choice for each of the following multiple choice questions (3 points each) 1. You attempt to amplify a gene using PCR and find that a sample of the PCR product gives multiple bands on a gel. To resolve what is most likely a problem of nonspecific priming you should a. Increase the temperature of the initial heating step that denatures the target DNA b. Decrease the temperature of the initial heating step that denatures the target DNA c. Increase the temperature of the step where the primers anneal to the target DNA d. Decrease the length of the primers e. Both c and d 2. The U tube apparatus was used to demonstrate that a. non-replicative transposition involves the physical movement of a transposon to a new location b. physical contact between bacteria is not required for transduction c. DNA replication is semiconservative d. physical contact between bacteria is required for conjugation e. both b and d 3. Which is true of an Hfr strain? a. Is less likely than an F+ strain to transfer E. coli genes to an F- strain b. Is formed by RecA dependent recombination between the F plasmid and host genome c. Only part of the F plasmid is contained within the host genome d. Is formed by conjugation between an F prime and an F+ strain e. both b and c 4. Which of the following would prevent you from determining the cotransduction frequency of two genes? a. The absence of a streptomycin resistance gene in the recipient bacterial strain b. The lack of information as to which was the “late” marker c. The absence of a chi site between the two genes d. The absence of a pseudo-pac site between the two genes e. The distance between the genes is greater than the length of the transducing phage genome 5. Which of these findings provided evidence for the formation of heteroduplex DNA during recombination? a. The presence of changed base pairs in the homologous DNA where the crossover took place b. The presence of changed base pairs in genetic markers flanking the region were the crossover took place c. The presence of mismatched bases in the region where the crossover event took place d. Electron microscopic images showing stem-loop structures at the location where the crossover took place e. The discovery of a transition mutation at the site where the recombination took place 6. You introduce a plasmid containing a nonreplicative transposon into an E. coli strain that contains a temperature sensitive allele of recA which yields an inactive RecA protein. Which of the following events would you expect to see when you shift the cells to the nonpermissive temperature? a. An increase in the frequency of transposition
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M101A_Winter2010Final - MCDB 101A Final Exam March 17, 2010...

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