MCDB_101A_F2010_Mid_2_key - MCDB 101A Midterm 2 100 points...

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1 MCDB 101A Midterm 2 Name______ KEY _____________________________ Nov 16, 2010 100 points total Perm#_______________________________________ Multiple Choice: Circle the letter corresponding to the best answer (3 points for each correct answer) 1. You find all white colonies on your plate after carrying out the blue-white cloning assay to determine which plasmid clones contain your gene. This could be due to the fact that a. a second recessive mutation has occurred within the C-terminal portion of the genomic copy of lacZ b. glucose is present in the growth media c. a mutation has occurred in the lacZ α peptide gene contained in the cloning plasmid d. b or c e. none of the above 2. Which of the following is true of polar mutations? 3. This method was used to demonstrate that the lac repressor protein binds to lacO DNA 4. Which of the following blotting techniques relies exclusively on capillary action to transfer the target molecule to a membrane for purposes of detection? 5. The ability to detect mismatches in homologous regions following recombination helped to validate the Holliday model. Function-disabling mutations in genes encoding which of the following proteins would help facilitate the detection of these mismatches? a. DNA Pol III b. DNA Pol I c. Mut S d. DNA adenine methylase e. All of the above 6. Shown to the right is a base pair that resulted from tautomerization. The bases are
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