MCDB_101A_F2010_Mid_2_key - MCDB 101A Midterm 2 100 points...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
1 MCDB 101A Midterm 2 Name______ KEY _____________________________ Nov 16, 2010 100 points total Perm#_______________________________________ Multiple Choice: Circle the letter corresponding to the best answer (3 points for each correct answer) 1. You find all white colonies on your plate after carrying out the blue-white cloning assay to determine which plasmid clones contain your gene. This could be due to the fact that a. a second recessive mutation has occurred within the C-terminal portion of the genomic copy of lacZ b. glucose is present in the growth media c. a mutation has occurred in the lacZ α peptide gene contained in the cloning plasmid d. b or c e. none of the above 2. Which of the following is true of polar mutations? a. They block translation of downstream genes encoded by a polycistronic mRNA b. They often act as frameshift repressor mutations c. They are associated with Rho-independent transcriptional termination d. Both a and b are correct e. Both b and c are correct 3. This method was used to demonstrate that the lac repressor protein binds to lacO DNA a. Northern blot b. Polymerase chain reaction c. Gel retardation assay d. Complementation test e. Western blot 4. Which of the following blotting techniques relies exclusively on capillary action to transfer the target molecule to a membrane for purposes of detection? a. Southern blotting b. Northern blotting c. Western blotting d. both a and b e. None of the above 5. The ability to detect mismatches in homologous regions following recombination helped to validate the Holliday model. Function-disabling mutations in genes encoding which of the following proteins would help facilitate the detection of these mismatches? a. DNA Pol III b. DNA Pol I c. Mut S d. DNA adenine methylase e. All of the above 6. Shown to the right is a base pair that resulted from tautomerization. The bases are a. enol cytosine and imino adenine b. keto guanine and enol adenine c. imino thymine and enol cytosine d. keto thymine and enol guanine e. pseudo uracil and amino xanthine
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
2 7. In the presence of IPTG, a merodiploid of genotype I -d P+O+ Z-Y+/I+ P-O C Z+Y- will produce a. Both β galactosidase and permease b. β galactosidase but no permease c. Permease but no β galactosidase d. Neither β galactosidase nor permease e. Blue colonies on X-gal media 8. The function of CAP is most similar to that of a. N protein b. integrase c. cII d. cIII e. DNA adenine methylase 9. (6 points) Match the proteins in column I with their functions in column II by writing the correct letter in each space. Some proteins may have multiple functions while others may not carry out any of the listed
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 02/18/2011 for the course MCDB 101A taught by Professor Thrower during the Spring '08 term at UCSB.

Page1 / 6

MCDB_101A_F2010_Mid_2_key - MCDB 101A Midterm 2 100 points...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online