Lab_3_Key.doc - io10DL B Winter2011 Lab3Key 1) 2,3,1,2,2 2)...

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Bio 10 DL                                                   Winter 2011                                                            Lab 3 Key     1) 2, 3, 1, 2, 2 2) 3, 4, 2, 3, 3 3) 22, 14, 8 6, 16, 14, 8 36, 8 6, 8, 30 6, 30, 8 4) From left to right the wells should be 3, 4, 5, 1, 2 or 3, 5, 4, 1, 2 5) You can not be sure of sequences 4 and 5, since they both present the same  number and pattern of bands when cut with the restriction enzyme used 6) You would have to use a different restriction enzyme to cut the DNA differently to  try to differentiate between sequences 4 and 5.  Or you could use a different  sequence of base pairs to distinguish between these 2 people.  Or, worst case  scenario, if you can’t ever distinguish between these 2 people, they’re identical  twins!! 7) The agarose gel acts like a sieve, with pores or spaces between the polymers of 
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This note was uploaded on 02/18/2011 for the course BIOL 010 taught by Professor Johnson during the Winter '08 term at DeAnza College.

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Lab_3_Key.doc - io10DL B Winter2011 Lab3Key 1) 2,3,1,2,2 2)...

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