lecture_17 - Enzyme Kinetics and Catalysis II Kinetics of...

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Enzyme Kinetics and Catalysis II 3/24/2003
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Kinetics of Enzymes Enzymes follow zero order kinetics when substrate concentrations are high. Zero order means there is no increase in the rate of the reaction when more substrate is added. Given the following breakdown of sucrose to glucose and fructose Sucrose + H 2 0 Glucose + Fructose O H HO H HO H OH OH H H OH OH HO H H OH O H H HO H H H OH
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P E ES S E 2 1 1 - k k k + → + E = Enzyme S = Substrate P = Product ES = Enzyme-Substrate complex k 1 rate constant for the forward reaction k -1 = rate constant for the breakdown of the ES to substrate k 2 = rate constant for the formation of the products
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When the substrate concentration becomes large enough to force the equilibrium to form completely all ES the second step in the reaction becomes rate limiting because no more ES can be made and the enzyme-substrate complex is at its maximum value. [ ] [ ] ES P 2 k dt d v = = [ES] is the difference between the rates of ES formation minus the rates of its disappearance.
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