lecture_22a - Glycolysis Pathway overview 1 Add phosphoryl...

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Glycolysis 5/9/03
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Pathway overview 1. Add phosphoryl groups to activate glucose. 2. Convert the phosphorylated intermediates into high energy phosphate compounds. 3. Couple the transfer of the phosphate to ADP to form ATP. Stage I A preparatory stage in which glucose is phosphorylated and cleaved to yield two molecules of glyceraldehyde-3- phosphate - uses two ATPs Stage II glyceraldehyde-3-phosphate is converted to pyruvate with the concomitant generation of four ATPs-net profit is 2ATPs per glucose. Glucose + 2NAD + 2ADP +2P i 2NADH + 2pyruvate + 2ATP + 2H O + 4H
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Front half of glycolysis
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Phosphoglycerate Kinase: First ATP generation step C CH 2 OPO 3 2- OH H O - O C CH 2 OPO 3 2- OH H OPO 3 2- O + ADP + AT P 1,3 BPG 3 PG
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GAP + Pi + NAD + 1,3 -BPG + NADH + 6.7 kJ• mol -1 1,3 BPG + ADP 3PG + ATP -18.8 kJ• mol -1 GAP+Pi+NAD + +ADP 3PG+NADH+ATP -12.1 kJ mol -1
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Phosphoglycerate mutase C CH 2 O PO 3 2- OH H O - O C CH 2 OH O PO 3 -2 H O - O C CH 2 O PO 3 2- O PO 3 -2 H O - O 3 PG 2PG 2,3 BPG
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Phosphoglycerate mutase requires a phosphorylated form of the enzyme to be active. Only 2,3 BPG can phosphorylate the unphosphorylated enzyme. N N H 2 C Enzyme PO 3 2- Phospho Histidine residue
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Glycolysis influences oxygen transport
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Oxygen saturation curves in erythrocytes
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Enolase generation of a second “high energy” intermediate C C OPO 3 -2 H O - O OH H H C OPO 3 -2 O - O H H 2 Phosphoglycerate Phosphoenol pyruvate
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Pyruvate kinase: Second ATP generation step
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The second half of glycolysis
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The metabolic fate of pyruvate
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The need to regenerate NAD + from NADH A. Homolactic fermentation: conversion of pyruvate to lactate Mammals have two different types of enzymes: Isozymes M type for muscle H type for heart C O - O O CH 3 N NH 2 O H R H S + + H + R C O - O CH 3 N H NH 2 O R H HO + LDH Pyruvate NADH L -Lactate NAD +
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Lactate dehydrogenase is a tetramer H4 has a low K m for pyruvate and is allosterically inhibited by high concentrations of pyruvate. M4 has a higher K
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This note was uploaded on 02/22/2011 for the course BCHS 3304 taught by Professor Johnson during the Spring '08 term at University of Houston.

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lecture_22a - Glycolysis Pathway overview 1 Add phosphoryl...

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