lecture_7legge - Chapter 4 (1) G A Proteins: Evolution, and...

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1 Proteins: Evolution, and Analysis Lecture 7 9/15/2009 Chapter 4 G A V L (1) I M P F W S T N (2) Chapter 4 Q Y C K R (3) H D E The Fischer Convention Absolute configuration about an asymmetric carbon related to glyceraldehyde (+) = D -Glyceraldehyde (-) = L -Glyceraldehyde Cahn - Ingold - Prelog system Can give absolute configuration nomenclature to multiple chiral centers. Priority Atoms of higher atomic number bonded to a chiral center are ranked above those of lower atomic number with lowest priority away from you R highest to lowest = clockwise, S highest to lowest = counterclockwise SH>OH>NH 2 >COOH>CHO>CH 2 OH>C 6 H 5 >CH 3 >H
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2 Newman Projection A projection formula representing the spatial arrangement of bonds on two adjacent atoms in a molecular entity. The structure appears as viewed along the bond between these two atoms, and the bonds from them to other groups are drawn as projections in the plane of the paper. The bonds from the atom nearer to the observer are drawn so as to meet at the centre of a circle representing that atom. Those from the further atom are drawn as if projecting from behind the circle. The major advantage of the CIP or RS system is that the chiralities of compounds with multiple asymmetric centers can be unambiguously described Structural Hierarchy in proteins Overview of Protein Sequencing (1) Purify Protein (2) Determine number of PP End group analysis (Dansyl chloride rxn) (3) Fragment PP into smaller peptides Enzymes (Trypsin, Chymotrypsin, etc.) Chemical (CNBr) (4) Determine the sequence Edman Degradation with PITC (5) Assemble a sequence (6) Elucidate S-S bonds Amino acid composition Long peptides have to be broken to shorter ones to be sequenced
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3 Q9 . You must cleave the following peptide into smaller fragments. Which of the proteases listed in the table would be likely to yield the most fragments? The fewest?
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This note was uploaded on 02/22/2011 for the course BCHS 3304 taught by Professor Johnson during the Spring '08 term at University of Houston.

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lecture_7legge - Chapter 4 (1) G A Proteins: Evolution, and...

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