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lecture_16legge - max = α o v ⎟ ⎟ ⎠ ⎞ ⎜ ⎜ ⎝...

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1 Enzyme Kinetics II 10/16/2008 Kinetic data cannot unambiguously establish a reaction mechanism. Although a phenomenological description can be obtained the nature of the reaction intermediates remain indeterminate and other independent measurements are needed . Reaction Mechanisms A: Sequential Reactions All substrates must combine with enzyme before reaction can occur Bisubstrate reactions Random Bisubstrate Reactions Ping-Pong Reactions Group transfer reactions One or more products released before all substrates added
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