lecture_16legge - max + = o v [ ] + = I K I 1 [ ][ ] [ ] EI...

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1 Enzyme Kinetics II 10/16/2008 Kinetic data cannot unambiguously establish a reaction mechanism. Although a phenomenological description can be obtained the nature of the reaction intermediates remain indeterminate and other independent measurements are needed. Reaction Mechanisms A: Sequential Reactions • All substrates must combine with enzyme before reaction can occur Bisubstrate reactions Random Bisubstrate Reactions Ping-Pong Reactions • Group transfer reactions • One or more products released before all substrates added
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2 Inhibition kinetics There are three types of inhibition kinetics competitive, mixed and uncompetitive. •Competitive- Where the inhibitor competes with the substrate. Competitive Inhibition [ ] [] S K S V M
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Unformatted text preview: max + = o v [ ] + = I K I 1 [ ][ ] [ ] EI I E K I = HIV protease inhibitors Competitive Inhibition: Lineweaver-Burke Plot 3 Uncompetitive Inhibition Uncompetitive Inhibition: Lineweaver-Burke Plot Mixed inhibition Mixed inhibition is when the inhibitor binds to the enzyme at a location distinct from the substrate binding site. The binding of the inhibitor will either alter the K M or V max or both. [ ][ ] [ ] EI I E K I = [ ] [ ] [ ] ESI I ES K I = [ ] [ ] S K S V M max + = o v [ ] + = I K I 1 4 The effect of pH on kinetic parameters Lecture 17 Tuesday 10/21/08 Enzyme Kinetics / Exam II Minireview...
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This note was uploaded on 02/22/2011 for the course BCHS 3304 taught by Professor Johnson during the Spring '08 term at University of Houston.

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lecture_16legge - max + = o v [ ] + = I K I 1 [ ][ ] [ ] EI...

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