Evaluation of cytokeratin 19 and cytokeratin 20 and interleukin 6 in

Evaluation of cytokeratin 19 and cytokeratin 20 and interleukin 6 in

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Egyptian bladder cancer patients 1 Eman Mahmoud El-Salahy Medical Biochemistry Dep., Ain Shams Faculty of Medicine, Cairo, Egypt Received 30 April 2002; accepted 13 September 2002 Abstract Objectives: This study was designed to detect the expression of CK-19 and CK-20 in tissue specimens and IL-6 in the sera (as a noninvasive maneuver) of bladder cancer patients. Results were correlated with the clinico-pathologic parameters, Bilharziasis and the occurrence of relapse of the carcinoma among Egyptian bladder cancer patients. Methods: Subjects of this study were 50 cases of bladder carcinoma (19 cases were positive for Bilharziasis) as well as 20 cystitis cases as control (7 cases were positive for Bilharziasis). Messenger ribonucleic acid extracted from fresh frozen tissue specimens with bladder tumor and the control group were collected and subjected to RT-PCR to detect expression of the amplification bands of CK-19 and CK-20 (214 and 370 base pairs). In the mean time, Interleukin-6 was quantified in the sera of the patients using ELISA kit. Results: CK-19 and CK-20 RNAs were expressed in bladder cancer cases, but not expressed in the control group. They were significantly correlated to advanced tumor stage and grade, while CK-19 positivity, was also, correlated to tumor recurrence and tumor pathology being more in SCC than TCC. Moreover, IL-6 positivity was correlated to the occurrence of malignancy, advanced grade and pathology being more in SCC than TCC. ROC curve was utilized to choose the best cut-off for serum IL-6 (49.2 pg/mL). At the determined cut-off, the sensitivity was 66% and the specificity was 95%. Bilharziasis was found to be related to advanced stages and grades of bladder cancer. Conclusion: CK-19, CK-20 and IL-6 were strongly associated with malignant phenotype of Egyptian bladder tissues, so they may be used as additional markers for assessment of bladder cancer patients. © 2002 The Canadian Society of Clinical Chemists. All rights reserved. Keywords: Cytokeratin 19; Cytokeratin 20; Interleukin-6; Reverse Transcriptase Polymerase Chain Reaction; Cystitis; Bilharziasis; Transitional Cell Carcinoma; Squamous Cell Carcinoma 1. Introduction Second to prostatic carcinoma, bladder carcinoma is the most frequent malignant tumor of the urinary tract and second most common malignancy of the genitourinary sys- tem [5]. Among Egyptians, bladder cancer is a major health prob- lem as it is the most common malignant disease [27]. It accounts for 29.85% of all malignant diseases from central registry [39]. Egyptian bladder cancer has two major types namely schistosomal bladder carcinoma and nonschistoso- mal carcinoma. The nonschistosomal tumors are similar to the western type of transitional cell carcinoma and associ- ated with exposure to certain occupational and environmen- tal carcinogens. On the other hand, schistosomal tumors are associated with schistosoma hematobium infestation of the
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This note was uploaded on 02/22/2011 for the course HTEC 50 taught by Professor Hassel,patricia during the Spring '11 term at DeAnza College.

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Evaluation of cytokeratin 19 and cytokeratin 20 and interleukin 6 in

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