2 - 2 with deletion of 9p21 locus, 25 with aneuploidy for...

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Unformatted text preview: 2 with deletion of 9p21 locus, 25 with aneuploidy for chromosomes 3, 7 and 17 and 2 with both clones. There were 2 false positive results between patients without bladder cancer, which indicate the specificity of 92%. Conclusion: Our results confirm high specificity and sensitivity of FISH in non- invasive bladder cancer detection espe- cially for high grade tumors. Detection of concrete chromosomal aberrations corre- lated with the grade of the tumor. Supported by grants IGA ND8095-3 and VZ MSM 0021620808 MP-12.15 The role of transforming growth factor-Beta1 in human bladder cancer cell lines Lee C 1 , Lee S 2 , Kim D 1 , Jeon Y 1 , Lee N 1 1 Soonchunhyang University Cheonan Hospital; 2 Soonchunhyang University College of Medicine, Cheonan, Korea Introduction: The transforming growth factor- b (TGF- b ) can trigger various cellu- lar responses including the inhibition of cell growth, differentiation, migration and apoptosis. In the present study, we would like to elucidate the role of TGF- b in blad- der cancer cells. Methods: The cell viability was observed at 24, 48 and 72 hours after treatment of TGF- b in bladder cancer cell lines - 253J, 5637, T24 and J82 - using MTT assay. And then we neutralize the effect of TGF- b using pan-TGF- b antibody and observed cell growth pattern again. The cell was exposed to TGF- b and we extracted total RNA at 6h and 12h after exposure for mi- croarray analyses. The microarray analyses were carried out using 22K oligochips. Results: Of the bladder cancer cell lines, 253J and 5637 showed growth inhibition after TGF- b treatment. Other two bladder cancer cell lines, T24 and J82 seemed not to be affected by TGF- b treatment. Aftertreatment....
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This note was uploaded on 02/23/2011 for the course HTEC 50 taught by Professor Hassel,patricia during the Spring '11 term at DeAnza College.

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