2 with deletion of 9p21 locus, 25 withaneuploidy for chromosomes 3, 7 and 17and 2 with both clones. There were 2false positive results between patientswithout bladder cancer, which indicatethe specificity of 92%.Conclusion:Our results confirm highspecificity and sensitivity of FISH in non-invasive bladder cancer detection espe-cially for high grade tumors. Detection ofconcrete chromosomal aberrations corre-lated with the grade of the tumor.Supported by grants IGA ND8095-3 andVZ MSM 0021620808MP-12.15The role of transforming growthfactor-Beta1 in human bladder cancercell linesLee C1, Lee S2, Kim D1, Jeon Y1, Lee N11Soonchunhyang University CheonanHospital;2Soonchunhyang UniversityCollege of Medicine, Cheonan, KoreaIntroduction:The transforming growthfactor-(TGF- ) can trigger various cellu-lar responses including the inhibition ofcell growth, differentiation, migration andapoptosis. In the present study, we wouldlike to elucidate the role of TGF-in blad-der cancer cells.Methods:The cell viability was observedat 24, 48 and 72 hours after treatment ofTGF-in bladder cancer cell lines - 253J,5637, T24 and J82 - using MTT assay. Andthen we neutralize the effect of TGF-using pan-TGF-antibody and observedcell growth pattern again. The cell wasexposed to TGF-and we extracted totalRNA at 6h and 12h after exposure for mi-croarray analyses. The microarray analyseswere carried out using 22K oligochips.Results:Of the bladder cancer cell lines,253J and 5637 showed growth inhibitionafter TGF-treatment. Other two bladdercancer cell lines, T24 and J82 seemed notto be affected by TGF-treatment. Afterneutralization of TGF-effect, 253J blad-
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