Study Guide - DNA Replication

Study Guide - DNA Replication - • Compare and contrast...

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Study Guide DNA Replication After these lectures you should be able to: Answer the end of the chapter questions from your textbook o Chapter 12 1, 5, 8, 9, 13, 23, 24, 25, 33 Explain why DNA is considered semi-conservative replication Describe the process of DNA replication, and explain the role of every enzyme and protein used in this process, including initiation at the origin of replication Distinguish between the leading strand and the lagging strand Explain how the lagging strand is synthesized when DNA polymerase can add nucleotides only to 3’ end Explain the functional and enzymatic differences between DNA Pol I and PolII Explain DNA polymerase editing
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Unformatted text preview: • Compare and contrast replication in prokaryotes and eukaryotes • Explain how replication of the telomeres is accomplished • Draw and label a replication fork with replicating strands, their direction, and the proteins WORD LIST bidirectional replication 5’→3’ exonuclease origin of replication Conservative replication 3’→5’ exonuclease primase Dispersive replication helicase RNA primer dnaA protein lagging strand proofreading DNA gyrase leading strand replication bubble DNA polymerase I ligase replication fork DNA polymerase III okazaki fragments semi-conservative dNTPs single strand binding (SSB) protein telomerase...
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