Study Guide - Transcription and Processing

Study Guide - Transcription and Processing - • Explain...

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Study Guide After these lectures, you should be able to: Answer the following questions from your textbook o Chapter 13 1, 4, 5, 9, 13, 20, 21, 28 o Chapter 14 12, 20, 21, 23, 28 Explain how information is passed from DNA to RNA to protein Describe a transcription unit Explain the process of transcription in a diagram (polarity/strand) Compare and contrast prokaryotic and eukaryotic promoters and critical proteins that bind the promoter. Compare and contrast prokaryotic and eukaryotic RNA polymerases, and inititation cofactors (sigma, TFIID) Compare and contrast basal transcription factors and transcriptional activator proteins Describe two ways in which bacterial transcripts are terminated and how the gene sequence determines which is used. Compare and contrast the roles of rho and rat1 in the termination of transcription
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Unformatted text preview: • Explain how eukaryotic mRNA is processed before it leaves the nucleus- including 5’CAP, poly A tail and splicing • Describe alternative splicing • Describe mRNA editing WORD LIST basal transcription apparatus non-coding strand TFIID/TATA binding protein central dogma precursor mRNA TATA box coding strand promoter template strand core promoter Poly A polymerase Rho termination factor Downstream (towards 3’) Rat1- termination in Eukaryotes terminator Elongation Rho-dependent termination transcription Enhancer Rho-independent termination transcription unit general transcription factors RNA polymerase tRNA Holoenzyme-RNA Polymerase rRNA Upstream (towards 5’) mRNA sigma factor ( σ 70 ) 5’UTR-Untranslated Region 3' poly A tail Exons 3' UTR precursor mRNA 5' cap splicing 5' UTR spliceosome...
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