20 21 - 20.1 DNA Replication The structure of the double...

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20.1 DNA Replication The structure of the double helix suggested a basic mechanism for DNA replication. One strand is the complement of the other and could serve as a template for its synthesis. Fundamental rules of DNA replication 1) DNA Replication is semiconservative
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20.2 2) DNA replication begins at an origin and proceeds bidirectionally (cell chromosomes) 3) DNA synthesis proceeds in a 5’ 3’ direction and is semi- discontinuous. Okazaki fragments found on ‘lagging strand.’
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DNA Polymerases 20.3 There are five known DNA polymerases in E. coli DNA polymerase I, II, III, IV and V General features of DNA polymerases Require a DNA template Require a primer with free 3’OH end (cannot initiation chain synthesis de novo) Synthesis requires dNTPs and Mg ++
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20.4 DNA Polymerase I: 3 enzymes in one polypeptide chain >5’-3’ polymerase (requires properly base paired 3’-terminus before next nucleotide added) >3’ 5’ exonuclease (proofreading activity that removes misincorporated
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This note was uploaded on 02/23/2011 for the course CHEM 4731 taught by Professor Mchenry during the Spring '08 term at Colorado.

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20 21 - 20.1 DNA Replication The structure of the double...

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