37 - RNA interference(RNAi 37.1 A conserved biological response to double-stranded RNA known variously as RNA interference(RNAi or

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Caenorhabditis elegans as a response to double-stranded RNA (dsRNA), which resulted in sequence-specific gene silencing. Fire, Mello and colleagues were attempting to use antisense RNA as an approach to inhibit gene expression. Their breakthrough was to test the synergy of sense and antisense RNAs, and they found that the dsRNA mixture was at least tenfold more potent as a silencing trigger than were sense or antisense RNAs alone. Exposure of a parental animal to only a few molecules of dsRNA per cell triggered gene silencing throughout the treated animal (systemic silencing) and in its F1 (first generation) progeny. A conserved biological response to double-stranded RNA, known variously as RNA interference (RNAi) or post-transcriptional gene silencing, mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes. RNAi has been cultivated as a means to manipulate gene expression experimentally and to probe gene function on a whole-genome scale. RNA interference (RNAi)
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This note was uploaded on 02/23/2011 for the course CHEM 4731 taught by Professor Mchenry during the Spring '08 term at Colorado.

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37 - RNA interference(RNAi 37.1 A conserved biological response to double-stranded RNA known variously as RNA interference(RNAi or

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