Chromatography1 - CHROMATOGRAPHY CHEM3001 - Chemical...

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CHROMATOGRAPHY CHEM3001 - Chemical Analysis II Donna A Minott-Kates
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DMK: CHEM3001 - Chromatography (2010-11) OBJECTIVES Review the principles of chromatography Examine sampling methods for chromatographic techniques Consider more advanced concepts of chromatographic separation techniques for the detection and identification of analytes
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DMK: CHEM3001 - Chromatography (2010-11) COURSE OUTLINE Review - principles of chromatography Sampling for chromatography Extraction/clean-up Headspace Derivitization GC General instrumentation Applications (temperature programming) Detectors LC HPLC, instrumentation Separation mechanisms columns, isocratic vs gradient elution Detectors Hyphenated systems eg GC-MS Other chromatographic systems
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DMK: CHEM3001 - Chromatography (2010-11) REFERENCES Skoog, D.A., West, D.M., Holler, F.J. and Crouch S.R. 2004. Fundamentals of Analytical Chemistry , 8th Edition. Brooks/Cole. Chapters 28-30. Harris, D.C. 2007. Quantitative Chemical Analysis , 7th Edition. W.H. Freeman and Company, New York. Chapters 22-26 Skoog, D.A., Holler, F.J. and Crouch S.R. 2006. Principles of Instrumental Analysis , 6th Edition. Brooks/Cole. Chapters 26-28
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DMK: CHEM3001 - Chromatography (2010-11) CHROMATOGRAPHY revision CHEM2001 History, definition, classification Band broadening, van Deemter GC instrument layout, FID LC TLC, gravity HPLC instrument layout, components Applications Qualitative t R , KI, Quantitative peak area vs height, normalisation, calibration curve, standard addition, internal standard
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CHROMATOGRAPHY terms Chromatograph : Instrument used to effect separation of analytes Chromatogram : Graphical representation (detector response vs time) of analyte separation Elution : Process of displacing analytes from a column Eluent : Fluid (mobile phase) used to partition and elute sample components Eluate : Fluid exiting column Retention : Absorption of analyte to stationary phase Retention Time : Time elapsed, t R , between injection of sample and detection of analyte peak maximum Dead Time : Time for non-retained species, t M , to traverse the chromatographic system and be detected
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Chromatography1 - CHROMATOGRAPHY CHEM3001 - Chemical...

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