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Lab2_Biotech_2 - DNA as the Hereditary Material The first...

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1 DNA as the Hereditary Material The first clue came from Griffith's discovery of transformation in Streptococcus pneumoniae . Griffith found that heat-killed virulent S. pneumoniae cells could transform a nonvirulent strain to virulence. What molecule within the heat-killed sample was carrying the information for virulence? Avery, MacLeod, and McCarty set out to determine whether protein, DNA, or RNA was responsible for the transformation. – Cell extracts from heat-killed virulent S. pneumoniae were treated with enzymes that selectively degraded DNA, RNA, or protein. – The extracts were then tested to see if they could still transform nonvirulent cells to virulence. Only extracts with intact DNA could transform cells to virulence, thus supporting the hypothesis that DNA is the hereditary material .
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2 The Hershey-Chase Experiment Hershey and Chase studied how the T2 virus infects the bacterium Escherichia coli . By radioactively labeling the virus's DNA with 32 P and its protein with 35 S, they demonstrated that DNA is the genetic material. DNA structure DNA is composed of deoxyribonucleotides . DNA is a polymer.
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