Introduction to Chemistry 241L

Introduction to Chemistry 241L - Chemistry 241L Laboratory...

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1 Chemistry 241L Laboratory in Separations and Analytical Characterization of Organic and Biological Compounds Fall 2010 Selected Text Taken From: Harris, Daniel C. Quantitative Chemical Analysis; 7th ed., W. H. Freeman & Company: New York, pp. 620-733. Christian, Gary D. Analytical Chemistry ; 5 th ed., John Wiley & Sons: New York, 1994, pp. 398-459. Used with permission.
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2 IMPORTANT NOTICE Below is a list of all the chemicals used in the Chemistry 241L lab. The Material Safety Data Sheets (MSDS) for all of these materials are on file in Morehead Labs Room 208. You can also find these sheets online at: http://msds.pdc.cornell.edu/msdssrch.asp If you are currently pregnant or become pregnant during the semester you are taking this course, you are encouraged to consult with your personal physician or contact Dr. Mary Schlegel at Student Health Services (966-2281) to discuss the advisability of continuing this course. Chemical Reagents Used In Chemistry 241L Acetic Acid Sodium Citrate, Tribasic Acetone Sodium Chloride Acetonitrile Sodium Fluoride Ammonium Chloride Sodium Hydrogen Sulfate Benzoic Acid Sodium Hydroxide Bovine Serum Albumin Sodium Phosphate, Dibasic Bromothymol Blue Sodium Phosphate, Tribasic Carbonic Acid Sodium Sulfate Citric Acid Sulfuric Acid Cytochrome c Trichloroacetic Acid Dextran Blue 2000 Dichloromethane Ethanol Ethyl Acetate Ferrocyanide β -D-Glucose Glucose Oxidase Hemoglobin Hexane Horseradish Peroxidase Hydrochloric Acid 1-Pentanol Phenol Red Ponceau Stain Potassium Hydrogen Phthalate Potassium Phosphate, monobasic Sephadex G-75 Sephadex G-25 Sodium Acetate Sodium Bicarbonate Sodium Carbonate
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3 INTRODUCTION TO CHEMISTRY 241L Introduction Welcome to Chemistry 241L. For some of you this will be your first laboratory course, and thus first laboratory experience at UNC. For others, this laboratory course will be a continuation of your chemical education in the laboratory, which started in Chemistry 101L and 102L. The focus of this laboratory course is to expose students to a wide variety of modern analytical methods and experimental techniques for the separation and characterization of organic and biological compounds. The laboratory skills and experimental techniques learned over the course of this semester will carry over to future courses you will take here at UNC. In addition, the analytical methods you will learn in this course are used in a wide variety of scientific fields and industries, such as medicinal chemistry, biochemistry, biotechnology, environmental science, nutritional analysis, chemical industries, and the pharmaceutical industry. A second important objective of this course is to teach students the importance of experimental work and expose them to the scientific process behind this work. Science is about curiosity, creativity, critical thinking, and discovery. Scientists observe, hypothesize, experiment, draw conclusions, and test their results. In this course students will be taught how to make accurate observations and measurements, and to learn how to interpret their results in a scientific manner.
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