Ch-20-Test-questions

Ch-20-Test-questions - Biology 8e(Campbell Chapter 20...

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Biology, 8e (Campbell) Chapter 20 Biotechnology Multiple-Choice Questions Figure 20.1 1) Which enzyme was used to produce the molecule in Figure 20.1? A) ligase B) transcriptase C) a restriction enzyme D) RNA polymerase E) DNA polymerase 2) Assume that you are trying to insert a gene into a plasmid. Someone gives you a preparation of genomic DNA that has been cut with restriction enzyme X. The gene you wish to insert has sites on both ends for cutting by restriction enzyme Y. You have a plasmid with a single site for Y, but not for X. Your strategy should be to A) insert the fragments cut with X directly into the plasmid without cutting the plasmid. B) cut the plasmid with restriction enzyme X and insert the fragments cut with Y into the plasmid. C) cut the DNA again with restriction enzyme Y and insert these fragments into the plasmid cut with the same enzyme. D) cut the plasmid twice with restriction enzyme Y and ligate the two fragments onto the ends of the DNA fragments cut with restriction enzyme X. E) cut the plasmid with enzyme X and then insert the gene into the plasmid. 3) What is the enzymatic function of restriction enzymes? A) to add new nucleotides to the growing strand of DNA B) to join nucleotides during replication C) to join nucleotides during transcription D) to cleave nucleic acids at specific sites E) to repair breaks in sugar-phosphate backbones 4) How does a bacterial cell protect its own DNA from restriction enzymes? A) adding methyl groups to adenines and cytosines B) using DNA ligase to seal the bacterial DNA into a closed circle C) adding histones to protect the double-stranded DNA D) forming "sticky ends" of bacterial DNA to prevent the enzyme from attaching E) reinforcing the bacterial DNA structure with covalent phosphodiester bonds 5) What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium? I. Transform bacteria with recombinant DNA molecule. II. Cut the plasmid DNA using restriction enzymes. III. Extract plasmid DNA from bacterial cells. 1
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IV. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments. V. Use ligase to seal plasmid DNA to nonplasmid DNA. A) I, II, IV, III, V B) II, III, V, IV, I C) III, II, IV, V, I D) III, IV, V, I, II E) IV, V, I, II, III 6) Bacteria containing recombinant plasmids are often identified by which process? A) examining the cells with an electron microscope B) using radioactive tracers to locate the plasmids C) exposing the bacteria to an antibiotic that kills cells lacking the resistant plasmid D) removing the DNA of all cells in a culture to see which cells have plasmids E) producing antibodies specific for each bacterium containing a recombinant plasmid Use the following information to answer the questions below. A eukaryotic gene has "sticky ends" produced by the restriction endonuclease EcoRI. The gene is added to a
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This note was uploaded on 02/25/2011 for the course BIO 1105 taught by Professor Dawkins during the Spring '10 term at McKendree.

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Ch-20-Test-questions - Biology 8e(Campbell Chapter 20...

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