Choose the one alternative that best completes the statement or answers the question.
1) For mapping studies of genomes, most of which were far along before 2000, the 3
was often used. Which is the usual order in which the stages were performed, assuming some
overlap of the three?
A) sequencing of entire genome, physical map, genetic map
B) linkage map, physical map, sequencing of fragments
C) genetic map, sequencing of fragments, physical map
D) cytogenetic linkage, sequencing, physical map
E) physical map, linkage map, sequencing
2) What is the difference between a linkage map and a physical map?
A) There is no difference between the two except in the type of pictorial representation.
B) For a linkage map, markers are spaced by recombination frequency, whereas for a physical
map they are spaced by numbers of base pairs (bp).
C) For a linkage map, it is shown how each gene is linked to every other gene.
D) For a physical map, the ATCG order and sequence must be achieved, but not for the linkage
E) For a physical map, the distances must be calculable in units such as nanometers.
3) How is a physical map of the genome of an organism achieved?
A) using recombination frequency
B) using very high
C) using DNA fingerprinting via electrophoresis
D) using sequencing of nucleotides
E) using restriction enzyme cutting sites
4) Which of the following most correctly describes a shotgun technique for sequencing a genome?
A) cloning several sizes of fragments into various size vectors, ordering the clones, and then
B) cloning the whole genome directly, from one end to the other
C) cloning large genome fragments into very large vectors such as YACs, followed by
D) physical mapping followed immediately by sequencing
E) genetic mapping followed immediately by sequencing
5) The biggest problem with the shotgun technique is its tendency to underestimate the size of the
genome. Which of the following might best account for this?
A) counting some of the overlapping regions of the clones twice
B) missing some duplicated sequences
C) missing some of the overlapping regions of the clones
D) having some of the clones die during the experiment and therefore not be represented
E) skipping some of the clones to be sequenced