Apr_09_2370

Apr_09_2370 - Name: _ Student Number: _ _ THE UNIVERSITY OF...

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Name: ________________________________ Student Number: __________________ _______________________________________________________________________________________ THE UNIVERSITY OF MANITOBA April 23, 2009, 9:00 AM -12:00 PM Page 1 (of 4) Paper # 649/651 Biochemistry II Laboratory Section Final Examination MBIO / CHEM.2370 Examiner: Dr. J. Galka _______________________________________________________________________________________ 1. Answer ALL questions in the space provided . 2. The back side of each page may be used for your answer or for preliminary work. 3. Questions to invigilators about the exam will NOT be answered . _______________________________________________________________________________________ Marks 5 1. A tissue extract containing six proteins, K, L, M, N, O, P, was dissolved in 0.15M phosphate buffer, pH 5.5. The molecular weights and isoelectric points of the proteins are shown below: Protein MW pI K 107,500 10.2 L 35,000 4.8 M 173,000 7.3 N 96,000 6.2 O 185,000 8.6 P 71,000 8.6 The tissue extract was placed on a CM-Sephadex column and subsequently eluted with three buffers to give three fractions (1A-3A). Fraction 1A was eluted at pH 5.5, 2A was eluted at pH 6.5, and 3A was eluted at pH 9.0. a) Indicate which, if any, of the six proteins would be found in these three fractions. Fraction 1A_______________________________________________________ Fraction 2A_______________________________________________________ Fraction 3A_______________________________________________________ b) Give reasons to explain your answers above, including a brief description of how the CM-Sephadex column functions, and why the proteins behave as they do.
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THE UNIVERSITY OF MANITOBA April 23, 2009, 9:00 AM -12:00 PM Page 2 (of 4) Paper # 649/651 Biochemistry II Laboratory Section Final Examination MBIO / CHEM.2370
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Apr_09_2370 - Name: _ Student Number: _ _ THE UNIVERSITY OF...

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