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Unformatted text preview: [ Name: Student Number: Page 1 (of 4) THE UNIVERSITY OF MANITOBA February 25, 2007, 6:00 PM Biochemistry H Mid-term Examination Time: 2 hours MBIO / CHEM 2370 Examiners: P. Loewen, J. Stetefeld 1. Answer ALL questions in the space provided. (You may use the back of the page if necessary.) 2. The exam will be marked out of 100 and count 25% of your final grade. 3. Questions during the exam period to invigilators about the exam will NOT be answered. Marks 20 1. Palmitic acid in which all hydrogen atoms are replaced with tritium (3H) is incubated with a cell extract capable of B-oxidation and the [3H] palmitic acid is completely metabolized to acetyl CoA. What is the 3I-I/C ratio in the acetyl CoA. To justify your answer, describe the pathway including all structural 1‘? formulae coenz es and enz e names. , c, f? A? amm- 3311?) FM) Fm“;- 1’" —— c ‘gCaA C _ Ta (C71), 1/ ‘ Michigan" can gm ““6““ T ‘podmduk isflfiéflul palm‘lmxl (of! dc Om we! M 94031 COP: craanCTL ‘SQR ”$01“ “00 flare W: ragga—€35 l, 107' L310 0 ( ‘ . C73 ~5-CTH~E~SCOA Owl *6: 3H / Q ”Amp or . (0 BUM (Ear/E XKCukS“ 5 2. Using structural formulae and names of enzymes and coenzymes describe the physiological basis of phenylketonuria. « adage.” in PLM (QLMC'Q kxolrox icy/AL r um (M Micki“! bdmj Ame waged if?“ (A: \M u.) c m ‘ reg Yfltfifl WW Ce“ dese\epm — pLfiauzscmvflWiQ February 25, 2007, 6:00 PM Biochemistry II 2370 Page 2 (of 4) Name: Student Number: Marks 15 3. Using structural formulae, including the “active” portions of all necessary coenzymes, describe the mechanism of oxidative decarboxylation of a—ketoglutarate catalde by a—ketoglutarate dehydrogenase. 7:/ \.i-_/ if“ \_/ \.._./ \ , / m ... —— E In 6 t "‘ N \ r0 k0 ls€ / / \ \ / +\\ /$P3P spkg’kl E E’XK /$ / IQI _c +\\ / C— c -———’> £3 E: __..—_~, Ho” whom-Lb?) *- t A v C\ C‘O: r [Er/C \jci H 0/ 9H; \5/ t L2,. H H 0 C147- Cldz \> /1_ \g cm can: Cu/ Em"? 1‘ 5:; (3,012” Kg” (3/? ‘3 C’JE - ~ , l d~l®r03lrdrwuk hdi I CHI. 0‘42. Cele KW Nkb'l cm i . I Coo“ ._ + 4; ’L /“ FAD \Q‘lw < \ SH 3'3 >_</ I .c “I / E com“ o 5% >5 Sucdvr l Cob ‘3 117—“ 4r. Calctfate the energy yield in ATP equivalents (assuming an active electron transport chain) when valine is degraded completely to C02. Justify your answers by using compound names only to describe the relevant pathways (but not the electron trans chain). Structures may be used in place of compound names. V M m: N kWh H" @ mm magma» a M? W -L Ft ,3 3 3 .4 Am ISOUaW-k CoPt 61.43% 0 lfifioétb C05 ’1) HOP L- / MMSM (05‘ Q\\ “MejcmuanUQ a» > 5mm “‘3 WW“ *‘3 Mala}: “7“ V ' Fhfimi‘ mot February 25, 2007, 6:00 PM Biochemistry 11 2370 Page 3 (of 4) Name: Student Number: Marks 20 5. Using structural formulae of all intermediates, and enzyme and coenzyme names, describe the pathway by which two aspartaé: moliiules can be converted into one molecule of glucose—6-phosphate.. ‘,. ko-o 5mm _ _ 3 $00 (ta-p 41$“? act? ‘ mow l\’/0"°i2!:>lf (£6 ((9 /blsp[x0$‘)l\k\‘0$9 gndo 1’ W “to“ EMU. 91 i? , high» Mia, 17%ka ’PLE “E":O $6) L» “I Wm‘zmmem We 3J0 Q F'FIVJ E anxfifggk H‘ O /%‘M [so-mun , [l' O __. OlJr 01+ 3W u’lo~rpkoevai-C 5 6. Using a diagram, explain how the oxidations of NADl-I and FADH2 are coupled to ATP production. Indicate in your diagram the yield of ATP per NADH and FADHZ. W4 aw T LH“ ‘In 7 V a wait-A am) We gradient is esinUCsi/ted Rig; \l ATP across’rCA mm mm a “eh; ?m wig/7 ashTP PH? MAME—‘9 10H+ PW FADHu-vbfl‘L Fwd /—> LSAT" February 25, 2007, 6:00 PM Biochemistry II 2370 Page 4 (of 4) Name: Student Number: Marks 8 7. Draw the reaction catalyzed by propionyl CoA carboxylase including the structures of biotin and its carboxylated derivative as well as the structures and names of the reactants and products. c2 _ (I? ‘00" c9 ' _. .- \ I) 3%:ESLOQ ' Cdz‘¢""¢"sc°’°'. (C A 09 \ {A t , '9 2.“ we WWW: 0 , o A“ ,LM» C” 3013 it; \I ”kl/6‘5)” \ 1 WWW“ ~ HC-— cfi H [C U. exp“, 1‘? by, C,“ \C'L 047:6]? ‘ Mix s/c‘ 56°- a it! ~0 \ r ' C13; b‘ + A Caribfisbw K 'D ‘ 8 8. Using structural formulae and names of all reactants and products as well as the names and active site structures of any coenzymes, describe the two reactions catalyzed by serine hydroxymethyl transferase. E9 Cm" “0'0, t l .. L t» . “2',“ "If“ ‘ tcfw ”s” ”i” ._—————————3 H 380m \ n N -Q.—H Cali 5 \ [+0 1 LO \4 CHLO it I! cal-:9 ~‘ _ Cris w-{CNH ~N/ t4» /ti~ *SQHLQ firewtmfl. ard'déxdfiiz % N. V skew /\_/)" +e,\foc\:o\wco\§c_ FM 051A Ft“: 9 9. Using structural formulae and the enzyme and coenzyme names, describe two separate reactions that bring about the interconversion of C3, C4, C5, C6 and C7 carbohydrates. What is the main purpose of the pathway containing these reactions? R? owl qua/0“ Q4 hushblatse Q :0 C.=O _‘ 430 ‘Crfl- 1 1 (4 C‘- 2 l H 0 -c.l\ so fat + [LC-:0 H a .L ,‘cxon t (Low - p t \ We 1 A ‘Q m H (5" H awafiw CW) 0'3: Cum/003 CH>°® Sacemmeja ole 5%:%‘0 ‘7‘ X\3Lr\osce S— Pbsphk n‘oo 3a“ S'pkosduk - 3.9km? Le $b6?k£ auto “t \ , t - :9 Chou I (L l “C"!g laz‘o - l l.” m l—O ,i,?,-o|¥ $9 HM 2:0“ t» #0 4&4? A0 -crur L H» front fie; ~ ée‘nDUD “(bolt {Jr -(Lml €441,009 p311; Phosphak Wk (9’ rt ‘ £411 \ - 1+ 3 C841 3 ‘ 026*»‘(9 See 61%” with - MAMQUAQ M1909 MWMW‘W "t ‘t huge-WW Cs’ Ca C3 CL M‘IKWUm‘elms out pax‘V 0Q ‘TCL Femflmimmcl PLuJL 0‘“ Ta ‘pew‘rosmykospm pajama?) $+mfiw¥m3 Q5 ear . ...
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