Apr_00_237 - Name: ________________________________ Student...

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Unformatted text preview: Name: ________________________________ Student Number: __________________ _______________________________________________________________________________________ THE UNIVERSITY OF MANITOBA April 10, 2000, 9:00 AM - 12:00 PM Page 1 (of 4) Paper # 2/3 Biochemistry II Lab Section Final Examination Microbiology/Chemistry 60.237/2.237 Examiner: Dr. A. Scoot _______________________________________________________________________________________ 1. Answer ALL questions. . 2. Questions must be answered in the space provided 3. The last page provides space for preliminary work. _______________________________________________________________________________________ Marks 1. Chromatographic methods are some of the most widely used techniques in biochemistry for separation and identification of components in a mixture. Name three of the four chromatographic methods used in the lab this term and include a brief explanation of how each method works. 3 3 3 THE UNIVERSITY OF MANITOBA April 10, 2000, 9:00 AM - 12:00 PM Page 2 (of 4) Paper # 2/3 Biochemistry II Lab Section Final Examination Microbiology/Chemistry 60.237/2.237 Examiner: Dr. A. Scoot _______________________________________________________________________________________ Marks 3 2. You are given a solution containing an enzyme that converts B into A. Outline briefly what you would do to determine the specific activity of this enzyme. 3 3. Answer ONE (1) of the following two parts, a or b. Explain the significance and mode of action of one of the two following sets of reagents in relation to their use in this terms biochemistry laboratories. (a) Barium hydroxide and arsenomolybdate solutions (used in same experiment) (b) Perchloric acid and potassium hydroxide (used in same experiment) THE UNIVERSITY OF MANITOBA April 10, 2000, 9:00 AM - 12:00 PM Page 3 (of 4) Paper # 2/3 Biochemistry II Lab Section Final Examination Microbiology/Chemistry 60.237/2.237 Examiner: Dr. A. Scoot _______________________________________________________________________________________ Marks 10 4. Two different columns were used to see which gave the better separation of lysozyme from a filtered buffered egg white preparation (FBEW). The lysozyme activity in 0.1 mL of a l in 2 dilution of FBEW wasThe lysozyme activity in 0....
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Apr_00_237 - Name: ________________________________ Student...

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