Apr_04_237 - Name: ________________________________ Student...

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Unformatted text preview: Name: ________________________________ Student Number: __________________ _______________________________________________________________________________________ THE UNIVERSITY OF MANITOBA April 13, 2004, 1:30 PM - 4:30 PM Page 1 (of 4) Paper # 138 / 139 Biochemistry II Lab Section Final Examination Microbiology/Chemistry 60.237/2.237 Examiner: Dr. A. Scoot _______________________________________________________________________________________ 1. Answer ALL questions in the space provided . 2. The back side of each page provides space for preliminary work. _______________________________________________________________________________________ Marks 12 1. You have a tissue homogenate containing three proteins: Protein A (MW 64,500 and pI 3.9), Protein B (MW 96,000 and pI 7.4) and Protein C (MW 185,000 and pI 8.8). Describe how you could separate these three proteins from each other using the three methods for protein separation used in the biochemistry lab this term. Your answer should include the name of each method, details of the principles behind each method and a brief description of materials and methods along with the expected results. Use a separate space for each method . Method #1 Method #2 Space for Method #2 continued THE UNIVERSITY OF MANITOBA April 13, 2004, 1:30 PM - 4:30 PM Page 2 (of 4) Paper # 138 / 139 Biochemistry II Lab Section Final Examination Microbiology/Chemistry 60.237/2.237 Examiner: Dr. A. Scoot _______________________________________________________________________________________ Marks Method #2 continued Method #3 THE UNIVERSITY OF MANITOBA April 13, 2004, 1:30 PM - 4:30 PM Page 3 (of 4) Paper # 138 / 139 Biochemistry II Lab Section Final Examination Microbiology/Chemistry 60.237/2.237 Examiner: Dr. A. Scoot _______________________________________________________________________________________ Marks 3 2. In the lab this term icing sugar and anhydrous petroleum ether were used in the same experiment. In the lab this term icing sugar and anhydrous petroleum ether were used in the same experiment....
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Apr_04_237 - Name: ________________________________ Student...

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