Apr_06_237 - Name: ________________________________ Student...

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Unformatted text preview: Name: ________________________________ Student Number: __________________ _______________________________________________________________________________________ THE UNIVERSITY OF MANITOBA April 10, 2006, 1:30 AM - 4:30 PM Page 1 (of 4) Paper # 64 / 65 Biochemistry II Laboratory Section Final Examination Microbiology/Chemistry 60.237/2.237 Examiner: Dr. A. Scoot _______________________________________________________________________________________ 1. Answer ALL questions in the space provided . 2. The back side of each page may be used for your answer or for preliminary work. 3. Questions to invigilators about the exam will not be answered . _______________________________________________________________________________________ Marks 2 1. State which chemicals or products were used in the lab this term for each of the following purposes. N.B. Full names and correct spelling required. A) To initially precipitate the RNA, DNA and protein from liver homogenate. Ans_____________________________________________________________________ B) To yield a purple complex upon reaction with peptide bonds in the assay to measure protein concentration. Ans_____________________________________________________________________ C) To form a coloured complex in the assay used to measure RNA concentration. Ans_____________________________________________________________________ D) To form a coloured complex in the assay used to measure DNA concentration. Ans_____________________________________________________________________ 4 2. Use a figure to illustrate the staining sequence used in the lab this term for lactate dehydrogenase. THE UNIVERSITY OF MANITOBA April 10, 2006, 1:30 AM - 4:30 PM Page 2 (of 4) Paper # 64 / 65 Biochemistry II Laboratory Section Final Examination Microbiology/Chemistry 60.237/2.237 Examiner: Dr. A. Scoot _______________________________________________________________________________________ Marks 7 3. Two methods of column chromatography were used to isolate glutamate dehydrogenase from a partially purified liver homogenate. partially purified liver homogenate....
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This note was uploaded on 02/27/2011 for the course MBIO 2370 taught by Professor Spearman during the Winter '11 term at Manitoba.

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Apr_06_237 - Name: ________________________________ Student...

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