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Lecture 2.notes - wavelengths are chosen properly 3...

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Lecture 2 1/20/11 Reading: Alberts 5th ed: Chapter 8 pg. 510-539.Chapter 9 pgs. 579-593;604- 608 1. Looking at cells in the Microscope a. what is resolution? b. what is numerical aperture? c. What is the limit of resolution? 2. Light Microscope can resolve details 0.2 microM apart. a. resolution: 0.2 microM b. Phase contrast and DIC microscopy c. Enhancement and analysis by Digital Techniques d. Fluorescence Microscopy: localize specific molecules i. Fluorescence Microscopy 1. uses antibodies (immunofluorescence microscopy) 2. uses GFP or variants of GFP (fixed or living cells) ii. Confocal Microscopy: sharp focus with optical sections iii. Multiple fluorescent labels can be used if excitation/emission
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Unformatted text preview: wavelengths are chosen properly 3. Electron Microscopy a. resolution: in practical terms, .1nm (1A) b. Immunoglold can localize specific molecules c. Scanning microscopy: a 3d image of the surface of a speciman d. Multiple images can be combined to increase resolution 4. Visualizing Molecules in Living Cells a. uses light microscopy b. Strategies to introduce Membrane-impermeant molecules into cells c. “caged” Molecules d. Fluorescent Proteins can be used to Tag Individual Proteins e. Light Can be Used to Manipulate microscopic Objects...
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