Lec8.2011 - Lecture 11 Golgi Transport the Trans-Golgi...

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Lecture 11 (02/10/2011). Golgi Transport, the Trans-Golgi Network Reading: Alberts, 5th Ed. Pages 779-798 I.  Vesicular transport within the Golgi complex - how do materials move through the Golgi ?   Experiment evidence for vesicle traffic A.  Maturation model - cisternae are transient structures; form at cis face by ER vesicle fusion, travel to trans face altered along the way; cisternae mature as move through  1. Evidence - algal covered by scales thus produce unusually large secretory products; seen within Golgi cisterna but not in small transport vesicles C.  Vesicular transport  cisternae remain in place as stable entities held together by protein scaffold; material  1. Evidence - cell-free reconstitution of vesicle transport in systems from Chinese hamster ovary (CHO) cells -  a. Donor fraction infected with vesicular stomatitis virus (VSV) that encodes integral protein (VSV-G) made in  D.Current thinking is both models are correct! II. Clathrin-coated vesicles: Bud from TGN and Plasma Membrane. A. Clathrin coats of these vesicles contain: 1. An outer honeycomblike lattice composed of clathrin (forms structural scaffold), and 2. An inner shell made of protein complexes (AP's, adaptor proteins or assembly proteins) -  B. On outer (cytosolic) surface, adaptors bind clathrin; holds clathrin scaffolding on vesicle surface 1. On inner surface, adaptors bind tails of membrane receptors
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This note was uploaded on 02/27/2011 for the course BIO 320 taught by Professor Staff during the Spring '08 term at University of Texas.

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Lec8.2011 - Lecture 11 Golgi Transport the Trans-Golgi...

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