Unformatted text preview: start- and stop-transfer signals give rise to the final arrangement. Eliminating the first start-transfer signal would not affect the function of the second transfer signal, which would initiate transfer of C-terminal segments just as it did in the unmodified, original protein. This conclusion is also reached by the fact that there are more than 100 amino acids at the N-terminus of the modified protein before the first transmembrane segment. The glycosylation sites near the N-terminus of the protein can only be modified if they are in the ER lumen. Thus, the mutant protein will not be glycosylated. Page 1 of 1...
View Full Document
- Spring '08
- cell biology, transmembrane segment, multipass transmembrane protein, hydrophobic transmembrane segment