Discussion Section 1

Discussion Section 1 - Discussion Section Proteins and...

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Discussion Section: Proteins and microscopy A protein complex has 3 subunits (or 3 individual proteins) that combine in a quaternary structure. + Add SDS Adding sds disrupts non-covalent bonds and coats the protein subunits in (-) charges - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - = covalent disulfide bond Add Beta-Mercaptoethanol = reduces (breaks) the covalent disulfice bonds
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- end + end If two proteins are the same size, they will migrate the same distance and look like a single band…. How can you distinguish between two proteins that migrate the same distance on a gel? If all three protein subunits are different sizes, then you will see three bands on the gel
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Homework problems Problem 4) The resolving power of a light microscope depends on the width of the cone of light that illuminates the specimen, the wavelength of the light used, and the refractive index of the medium separating the specimen from the objective and condenser lenses, according to the formula: resolution = 0.61 λ n sin θ where λ equals the wavelength of light used, n is the refractive index, and θ is half the angular width of the cone of rays collected by the objective lens. Assuming an angular width of 120° ( θ = 60°), calculate the various resolutions you would expect if the sample were illuminated with violet light ( λ = 0.4 µm) or red light (
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This note was uploaded on 02/27/2011 for the course BIO 320 taught by Professor Staff during the Spring '08 term at University of Texas.

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Discussion Section 1 - Discussion Section Proteins and...

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