Discussion Section 4 - Golgi membrane. A unique...

Info iconThis preview shows pages 1–7. Sign up to view the full content.

View Full Document Right Arrow Icon
63 KDa 94 KDa 10 KDa 10 KDa 13 KDa 36 KDa 3 KDa 3 KDa 19 KDa What would your gel look like if you Add Salt only 100 60 45 30 23 15 5
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
63 KDa 94 KDa 10 KDa 10 KDa 13 KDa 36 KDa 3 KDa 3 KDa 19 KDa What would your gel look like if you Add a Detergent 100 60 45 30 23 15 5
Background image of page 2
63 KDa 94 KDa 10 KDa 10 KDa 13 KDa 36 KDa 3 KDa 3 KDa 19 KDa What would your gel look like if you Add Trypsin then Detergent 100 60 45 30 23 15 5 Trypsin
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
94 KDa 10 KDa 10 KDa 13 KDa 36 KDa 3 KDa 3 KDa 19 KDa What would your gel look like if you permeate, add Trypsin, then add Detergent 100 60 45 30 23 15 5 Trypsin 63 KDa
Background image of page 4
GEF Sar1 GDP Sar1 GTP GTP Sar1 GTP Sar1 GDP Sar1 GTP Coat has GAP COPII coat proteins ER Lumen Cytosol
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Problem 1) Correct the following description, as necessary (what sentences are wrong and how would you make them right?): "Sar1 protein is a COPlI-recruitment GTPase that facilitates the unidirectional transfer of COPlI vesicles from the ER membrane to the
Background image of page 6
Background image of page 7
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Golgi membrane. A unique directionality is imposed on the transfer by the locations of a guanine-nucleotide exchange factor (GEF) and a GTPase-activating protein (GAP). The GEF, which is located in the ER membrane, stimulates vesicle formation by mediating attachment of Sar1-GTP to the ER membrane, where it recruits COPlI subunits. The GAP, which is located in the Golgi membrane, stimulates vesicle docking by stimulating hydrolysis of Sar1-bound GTP. Sar1-GDP causes disassembly of the COPlI coat, which prepares the vesicle for fusion with the Golgi membrane." Cis Golgi more acidic KDEL COPII KK = signal on tail of KDEL receptor to take it back to ER KK COPI...
View Full Document

Page1 / 7

Discussion Section 4 - Golgi membrane. A unique...

This preview shows document pages 1 - 7. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online