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Discussion Section 4 - Golgi membrane A unique...

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63 KDa 94 KDa 10 KDa 10 KDa 13 KDa 36 KDa 3 KDa 3 KDa 19 KDa What would your gel look like if you Add Salt only 100 60 45 30 23 15 5
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63 KDa 94 KDa 10 KDa 10 KDa 13 KDa 36 KDa 3 KDa 3 KDa 19 KDa What would your gel look like if you Add a Detergent 100 60 45 30 23 15 5
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63 KDa 94 KDa 10 KDa 10 KDa 13 KDa 36 KDa 3 KDa 3 KDa 19 KDa What would your gel look like if you Add Trypsin then Detergent 100 60 45 30 23 15 5 Trypsin
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94 KDa 10 KDa 10 KDa 13 KDa 36 KDa 3 KDa 3 KDa 19 KDa What would your gel look like if you permeate, add Trypsin, then add Detergent 100 60 45 30 23 15 5 Trypsin Trypsin 63 KDa
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GEF Sar1 GDP Sar1 GTP GTP Sar1 GTP Sar1 GDP Sar1 GTP Coat has GAP COPII coat proteins ER Lumen Cytosol
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Problem 1) Correct the following description, as necessary (what sentences are wrong and how would you make them right?): "Sar1 protein is a COPlI-recruitment GTPase that facilitates the unidirectional transfer of COPlI vesicles from the ER membrane to the
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Unformatted text preview: Golgi membrane. A unique directionality is imposed on the transfer by the locations of a guanine-nucleotide exchange factor (GEF) and a GTPase-activating protein (GAP). The GEF, which is located in the ER membrane, stimulates vesicle formation by mediating attachment of Sar1-GTP to the ER membrane, where it recruits COPlI subunits. The GAP, which is located in the Golgi membrane, stimulates vesicle docking by stimulating hydrolysis of Sar1-bound GTP. Sar1-GDP causes disassembly of the COPlI coat, which prepares the vesicle for fusion with the Golgi membrane." Cis Golgi more acidic KDEL COPII KK = signal on tail of KDEL receptor to take it back to ER KK COPI...
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