Lec2.2011.notes - Alberts, Q9-3 Variants of GFP: a Rainbow...

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Alberts, 9-1
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Alberts, 9-3 Light Microscope
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Alberts, 9-6
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Alberts, 9-7 Alberts, 9-11a
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Alberts, 9-7
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Alberts, 9-8 Four Types of Light Microscopy Bright Field Phase Nomarski or DIC Dark Field
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EM Immunofluorescence Alberts, 9-17
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Alberts, 9-13 Fluorescence Microscope: Optics
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Alberts, 9-18 Detecting Proteins for Immunofluorescence
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EM Immunofluorescence Alberts, 9-17
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Alberts, 9-24 GFP: Green Fluorescent Protein
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Tagging Proteins with the Green Fluorescent Protein Gene for GFP Gene for Protein of Interest DNA mRNA Protein GFP Protein of Interest
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Microtubule Capping Proteins GFP-EB1 The protein EB1 binds to GTP-tubulin at the plus-ends of microtubules
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Unformatted text preview: Alberts, Q9-3 Variants of GFP: a Rainbow of Colors! Alberts, 9-14 For every fluorescent molecule the Emission wave is longer than the Excitation wave Fluorescent Proteins & Dyes Multiple Fluorescent Probes can be used in a single cell Alberts, 9-15 Confocal Fluorescence Microscopy Alberts, 9-20 Confocal Fluor. Microscopy Conventional Fluor. Microscopy Alberts, 9-21 Optical Sections visualized by Confocal Fluor. Microscopy Alberts, 9-22 Alberts, 9-6 Alberts , 9-42 Light vs. Electron Microscope Alberts , 9-45 Alberts , 9-50 Fig 9-9 Image Processing...
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Lec2.2011.notes - Alberts, Q9-3 Variants of GFP: a Rainbow...

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