lec8.2011.handout - Lecture 8 TrafficintheGolgi

Info iconThis preview shows pages 1–8. Sign up to view the full content.

View Full Document Right Arrow Icon
Lecture 8 Traffic in the Golgi Decisions at the Trans-Golgi Network Lysosomes Signals on Proteins determine Route 
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
How do ER resident proteins remain in ER? 1. What ARE ER resident proteins? 1. Hypothesis: Signal sequence on resident ER proteins acts to traffic proteins back to the ER 3. How would you identify this signal sequence?
Background image of page 2
Common sequences is consistent with hypothesis Signal sequence on resident ER proteins acts to traffic proteins back to the ER Is this signal sequence necessary for sorting? Is this signal sequence sufficient for sorting?
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Demonstration that a signal sequence is sufficient for targeting -------------------------------------
Background image of page 4
Retrieval of  ER Proteins Soluble ER Proteins have KDEL at their c-terminus These bind the KDEL Receptor KDEL Receptor packaged into COPI-Coated vesicles For cis-Golgi--->ER transport At the Cis-golgi: 13-24
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Retrieval of ER Proteins Karp 8.25 Soluble ER Proteins have KDEL at their c-terminus These bind the KDEL Receptor KDEL Receptor packaged into COPI-Coated vesicles For cis-Golgi--->ER transport
Background image of page 6
Retrieval of ER Proteins 13-24
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 8
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 27

lec8.2011.handout - Lecture 8 TrafficintheGolgi

This preview shows document pages 1 - 8. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online