Lecture5.2011.notes - Membrane Traffic*Secretory Traffic...

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Membrane Traffic ** Secretory Traffic Endocytic Traffic
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Fig 12-40
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Fig 12-40
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Free vs. bound ribosomes Cooper 9.3 How do “bound” ribosomes bind the RER?
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Signal Sequences Alberts, 2002
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ER  Signal sequence of Growth Hormone:  Targeting Signal to the  RER Cooper 9.6 Most ER signal sequences: an amino-terminal stretch of nonpolar (hydrophobic) residues accompanied by basic residues (e.g. Arg.) Other examples:
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Signal Recognition Particle (SRP) 6 protein subunits of which one has GTPase activity, 1 small RNA subunit See Alberts 12-39
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• Multimeric protein complex that creates an aqueous channel in the ER membrane • Permeability has to be tightly regulated--> plug • conserved mechanism The translocon
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A secreted protein --nascent peptide to the RER Alberts 12-40 1. Signal peptide emerges - binds SRP Translation STOPS
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A secreted protein --nascent peptide to the RER 2. Attachment of the Ribosome to the ER: SRP-receptor + Translocon Fig 12-40
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A secreted protein --nascent peptide to the RER 1. The signal peptide binds the translocon 2. the channel opens Alberts 12-40
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A secreted protein --nascent peptide to the RER 4. SRP is released from the receptor 5. Polypeptide translocates through the channel Alberts 12-40
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A secreted protein --nascent peptide to the RER: Signal peptidase cleaves Alberts 12-45
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Synthesis of a Secreted Protein
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Transmembrane domain of GlycophorinA, an IMP How are the 20 amino acids (hydrophobic) inserted into a membrane?
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Transmembrane domain of GlycophorinA, an IMP How are the 20 amino acids (hydrophobic) inserted into a membrane?
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Secretory proteins are inserted with different orientations into ER--- Cooper 9.9 HOW?
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