mutant list-Spring 2011

mutant list-Spring 2011 - D101N Proposed function Mutagenic...

Info iconThis preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon
D101N Proposed function: Mutagenic oligonucleotide: (36 mer) 5' GGT TGC TGA TGC AGC CGA ATT C GT GAC GTA GTC CGG 3' 3'——————TAA——————5' Restriction endonuclease: EcoR I Site: 5' GAATTC # Sites in wild type: 3 # Sites in mutant: 4 Size of fragment: 430 bp Sequencing primer: AP732 Special instructions: Run 1.2% agarose gel.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
S102C Proposed function: Mutagenic oligonucleotide: (30 mer) 5' GGT TGC TGA TGC TGC GCA GTC GGT GAC GTA 3' 3'——————CGU——————5' Restriction endonuclease: Fsp I Site: 5' TGCGCA # Sites in wild type: 2 # Sites in mutant: 3 Size of fragment: 2149 bp Sequencing primer: AP732 Special instructions: NONE
Background image of page 2
D153A Proposed function: Mutagenic oligonucleotide: (25 mer) 5' CGG GCG TGG C TG C C T GCA ACT CTG C 3' 3'——————AC G——————5' Restriction endonuclease: Fok I Site: GGATG # Sites in wild type: 7 # Sites in mutant: 6 Size of fragment: 1.3 kb Sequencing primer: AP941 Special instructions: Run a 1.2% agarose gel.
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 4
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 03/01/2011 for the course BCHM 464 taught by Professor Staff during the Spring '08 term at Maryland.

Page1 / 8

mutant list-Spring 2011 - D101N Proposed function Mutagenic...

This preview shows document pages 1 - 4. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online