Roach3 lab manual

Roach3 lab manual - PSYC 401 LAB MANUAL! LAB 5 - ESCAPE...

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PSYC 401 LAB MANUAL LAB 5 - ESCAPE CIRCUIT PAGE 1 Core Questions What is conduction velocity and how do you measure it? What is a neural circuit ‘timing map?’ How do you trace a neural circuit? What are pin electrodes and how do they work? What are useful strategies for using combinations of electrodes? How do you manipulate the geometry of recording equipment to allow use of multiple electrodes? How do you decide whether or not to trust your data traces? Neural Circuit for Escape Timing is critical at each stage of CNS escape circuits WHAT ARE WE GOING TO DO? You will use a range of neural recording techniques to study aspects of the CNS processing of wind information leading to escape. Your ultimate goal is to make a timing map for all the components of the circuit. Web resources: Basic cockroach info with lots of links Cockroach FAQ Cockroach-inspired technology For fun - be sure to explore One of the goals of a neuroscientist when studying the control of behavior is to ‘map’ the neural circuit. This has two components: anatomy (Where does the information travel? What neurons are involved?); and function (How do the neurons in the pathway interact with one another?) Further, we are interested in ‘adaptiveness‘ — How is the neural circuit designed to optimize the speci±c behavior it controls? Because speed is critical to successful escape, we will focus on the timing of each step in the cockroach wind-triggered escape circuit. By strategic recording using combinations of electrodes, you can clock the signals traveling from ±liform hairs to leg muscles.
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PSYC 401 LAB MANUAL LAB 5 - ESCAPE CIRCUIT PAGE 2 THE SURGERY THE BASIC IDEA (THE FIGURE ON PAGE 7 WILL HELP) You now know what the wind-triggered responses in the abdominal connective look like, how many units are involved, how the response changes with intensity, and the lateralization characteristics of the response. The goal this week is to make a ‘timing map’ for the neural circuit that controls escape. That means determining the speed with which signals travel over the longer parts of the circuit and the latencies at each step of the way from displacement of the ±liform sensilla to leg movement. We know the behavioral latency: average of 54 ms for a stationary roach, but as fast as 14 ms when the roach is walking slowly. Presumably (hopefully . ..), the sum of all the neurophysiological latencies you determine will be shorter than those. How much shorter depends on biomechanical and inertial forces, i.e. how long after the muscles start to contract does the body part actually start moving? and how long after that does the roach’s body actually begin to turn? Even though we won’t be doing intracellular
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This note was uploaded on 03/01/2011 for the course PSYC 401 taught by Professor Yager during the Spring '11 term at Maryland.

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Roach3 lab manual - PSYC 401 LAB MANUAL! LAB 5 - ESCAPE...

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