RSA 2-11- 2008 Chaptre 5

RSA 2-11- 2008 Chaptre 5 - Lecture 9 Gene Mapping Chapter 5...

Info iconThis preview shows pages 1–15. Sign up to view the full content.

View Full Document Right Arrow Icon
Lecture 9 Gene Mapping Chapter 5
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Life Cycle of Chlamydomonas Form diploid cells (zygotes) by the fusion of reproductive cells (gametes or isogametes when “look” identical)
Background image of page 2
Life Cycle of Saccharaomyces cerevisiae
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Life Cycle of Neurospora
Background image of page 4
Life Cycle of Neurospora
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Ordered tetrads allow mapping a gene in relation to the centromere
Background image of page 6
Linkage Analyses in Haploid Organisms Neurospora: Model organisms for understanding the mechanism of recombination. All four haploid products of meiosis are contained in ascus Ordered asci (singular ascus) Sacks containing 8 spores in a string Each spore has genetic information from one single strand of a DNA duplex from each tetrad in the original zygotes Ascospores within ascus germinate into haploid individuals Saccharaomyces cerevisiae – bakers yeast Neurospora crassa – bread mold
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Three ways in which different ascospore patterns can be generated in Neurospora
Background image of page 8
Segregation patterns in ordered asci
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Segregation patterns in ordered tetrads
Background image of page 10
First Division Segregation 1. a a + + 2. + + a a Second Division Segregation 3. a + a + 4. + a + a 5. + a + + 6. a + + a Data: First Division = 65 Second = 70
Background image of page 11

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Calculating Distance betwee gene and the centromere ½ (second division segregant asci) D = X 100 Total asci Scored First division Segregants = 65 Decond Division Segregants = 70 (1/2) (70) D = X 100 = 25.9 135
Background image of page 12
Problem : In a cross in Neurospora involving two alleles, B and b, the following tetrad pattern were observed: BBbb 36 bbBB 44 BbBb 4 bBbB 6 BbbB 3 bBBb 7 Calculate the Distance between the gene and the centomere
Background image of page 13

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
on separate chromosomes
Background image of page 14
Image of page 15
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 04/04/2008 for the course BIO 2204 taught by Professor Athwal/haines during the Spring '08 term at Temple.

Page1 / 42

RSA 2-11- 2008 Chaptre 5 - Lecture 9 Gene Mapping Chapter 5...

This preview shows document pages 1 - 15. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online