Thin Layer Chromatography - V109 THIN LAYER CHROMATOGRAPHY...

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V109 G e n Ch e mLab Thin Lay e r Chromatography 1 Monmouth U THIN LAYER CHROMATOGRAPHY OBJE C TIV E: Students will learn the basic principles of chromatography and use them to identify the components of an unknown dye mixture through the use of Thin Layer Chromatography (TLC). INTRODUC TION In this experiment you will receive an unknown mixture containing two or three of the five dyes whose structures and names are shown below. N N N H 3 C H 3 C N N N H 3 C H 3 C O OH N N N N OH N N O H 3 C Butter Yellow Indophenol Blue Sudan I Solvent Red I Azobenzene <RX ZLOO EH DEOH WR LGHQWLI\ ZKLFK RI WKH G\HV LV LQ \RXU XQNQRZQ PL[WXUH E\ ³VSRWWLQJ´ a series of dyes and dye mixtures of known composition on the same thin layer chromatography plate and developing it. The method you will be using, thin layer chromatography (TLC), is widely used in chemistry laboratories and is illustrative of the wide variety of chromatographic methods used in chemistry, biology, and forensic science. Chromatography is a method of separation that depends on the distribution and re- distribution of materials between two phases, a mobil e pha s e and a s tationary pha s e . In our experiment the stationary phase is a solid and the mobile phase is a liquid. If two compounds A and B have different solubilities in the liquid phase and/or different tendencies to be adsorbed onto the solid surface, it is possible to separate them from one another as the mobile phase moves past the stationary phase. In our TLC experiment, we employ a solid, white material (silica gel) as a stationary
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This note was uploaded on 02/28/2011 for the course CHEM 112 taught by Professor Bradleyj.ingebrethsen during the Spring '05 term at Monmouth University-West Long Branch.

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Thin Layer Chromatography - V109 THIN LAYER CHROMATOGRAPHY...

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