Lecture_2_-_2011 - Microbial Genomes Features Analysis Role...

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Microbial Genomes Features Analysis Role of high-throughput sequencing Yeast - the eukaryotic model microbe Databases NCBI Microbial Genomes JGI (IMG) Wiki sites
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Genome of the week - Haemophilus influenzae First microbial genome completely sequenced. Took one year from start to finish. ( E. coli took 7). First genome sequenced by the shotgun method. Sequenced at TIGR in 1995. Started 4 years after the E. coli genome sequencing project and finished 2 years ahead!
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E. coli nucleoid
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Microbial Genome - old view ( E. coli ) Single chromosome. Circular. Replication initiates from a single fixed origin. Little or no horizontal gene transfer.
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Microbial Genome - new view Bacteria can contain more than one chromosome. Bacterial chromosomes can be linear. Horizontal gene transfer is common! Expect the unexpected.
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Features of microbial genomes 4 bases -A,G,C,T
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Establishing gene function - bioinformatics can only get you so far Book - claims that over 75% of genes can be assigned function. Genomes in table 1.4 - doesn’t support this claim. Many annotations suggest biochemical role, not biological function. Many annotations need to be verified experimentally. Some annotations are wrong.
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Why sequence so many microbial genomes? Develop technology for human genome project Examine the genomes of a wide range of microbes Novel drug/vaccine targets Identify new agricultural and industrial important enzymes Comparative genomics/evolution Sequence microbes that have no genetics
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This note was uploaded on 03/01/2011 for the course MMG 433 taught by Professor Britton,r during the Spring '08 term at Michigan State University.

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Lecture_2_-_2011 - Microbial Genomes Features Analysis Role...

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