Lecture_4_2011 - Genome of the week Bacillus subtilis...

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3/2/11 Genome of the week Bacillus subtilis Gram-positive soil bacterium Genetically tractable, well-studied Developmental pathways (sporulation, genetic competence) Industrial and agricultural importance 4.2 Mb genome (sequence completed 1997) Close relative of Bacillus anthracis (Anthrax)
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3/2/11 B. subtilis genome features 4,106 protein coding genes 10 rRNA operons Nearly 50% of the genome consists of paralogous genes. 77 ABC transporters (each contains 5 proteins) 10 phage like regions - horizontal transfer. Low GC regions in the genome. 18 sigma factors. 34 two-component regulatory systems.
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3/2/11 The addition of a nucleotide during DNA synthesis results in the liberation of PP. Long homopolymeric tracts are difficult for 454 to accurately read. Why?
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Click to edit Master subtitle style 3/2/11 454 video http://www.youtube.com/watch?v=kYAGFrbGl6E
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Click to edit Master subtitle style 3/2/11 Illumina video http://www.youtube.com/watch?v=HtuUFUnYB9Y
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3/2/11
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3/2/11
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3/2/11
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3/2/11
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3/2/11
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3/2/11 Advantages Can generate over 1 Gb of DNA in a single day. Price per base is much cheaper. Valuable for resequencing genomes and de novo sequencing of bacterial genomes (metagenome chromosome assembly) Disadvantages Not yet able to sequence genomes de novo (close).
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3/2/11 Emerging sequencing technologies SMRT – Pacific Biosciences – Single molecule detection – directly watch DNA polymerase incorporate DNA.
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This note was uploaded on 03/01/2011 for the course MMG 433 taught by Professor Britton,r during the Spring '08 term at Michigan State University.

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Lecture_4_2011 - Genome of the week Bacillus subtilis...

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