135 Problem 1 - o C Describe the preparation of 1.00 L of...

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Problem 1 (Due September 15) HEPES, N-2-hydroxyethylpiperazine-N’-ethanesulfonic acid, is a commonly used component of physiological buffers. The pK a of the protonated amino group of HEPES is 7.55 at 25
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Unformatted text preview: o C. Describe the preparation of 1.00 L of 0.100 M HEPES buffer, pH 8.00, from the sodium salt of the basic form of HEPES (MW 260.3), 6.0 M HCl and deionized water....
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