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Unformatted text preview: The following data was gathered in attempt to sequence an oligopeptide: 1) Complete acid hydrolysis: (B, 2C, F, G, I, K, L, M, P, R, S) 2) One cycle of Edman degradation: L, S 3) Carboxypeptidase A treatment (sufficient time to remove one a.a. per chain): D 4) Treatment with DTT and iodoacetate followed by Trypsin hydrolysis (the letters inside the parentheses represent the a.a. composition of the fragments; modified cys are represented by C): (R, S), (D,M), (C, F, G, I, L, P), (C, K) 5) Treatment with DTT and 2-bromoethylamine followed by Trypsin hydrolysis: (R, S), (D, M), (C ), (C, G, L), (F, I, P), (K) 6) Treatment with Chymotrypsin: No fragments 7) Treatment with Pepsin: (2C, D, G, K, L, M, R, S), (F, I, P) Provide the primary structure of the oligopeptide, showing the reasoning that led to your final result....
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This note was uploaded on 03/03/2011 for the course CHEM 135 at San Jose State University .