Problems 10, 11, and 12

Problems 10, 11, and 12 - Problem 10 a Anaplerotic...

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Problem 10 a) Anaplerotic reactions permit the TCA cycle to supply intermediates to biosynthetic pathways while maintaining the proper levels of cycle intermediates. Show the sequence of reactions which result in the net synthesis of citrate from pyruvate (one of which is a prominent example of an anaplerotic reaction). b)When the rate at which the TCA cycle operates slows, the concentration of acetyl CoA increases. Specify the effect of this metabolite on the activity of pyruvate carboxylase, and discuss the metabolic advantages this affords the cell if i) the TCA cycle is slowed due to depletion of intermediates, or ii) the cycle is slowed due to an abundant energy supply (i.e., high [ATP], [NADH]). Problem 11 A suspension of mitochondria is incubated with pyruvate, malate and 14 C-labeled triphenylmethylphosphonium (TPP) chloride under aerobic conditions. (Recall that TPP is a membrane-permeable lipophilic cation.) After a short incubation, the mitochondria are rapidly collected by centrifugation and their total
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Problems 10, 11, and 12 - Problem 10 a Anaplerotic...

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