Reworded Answers for Finals

Reworded Answers for Finals - F all 2000 : 2b. Each...

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Fall 2000 : 2b. Each molecule of glycerol produces 1 ATP while each molecule of glucose produces 2 ATPs. Hence, two molecules of glycerol will give the same amount of ATP as one molecule of Glucose. Each cycle of Anaerobic fermentation produces one molecule of NAD+ but we require two molecules of NAD+ for processing glycerol. Therefore, anaerobic fermentation CANNOT maintain the Redox-balance. Fall 2001 : 4. For anaerobic fermentation to take place, we need net production of ATP and no net consumption of metabolite required. Only Glyceraldehyde is able to fulfill these requirements. Even though it uses one ATP but it generates two ATPs in the end. Glycerol uses two molecules of NAD+ and generates only one molecule of it and hence NAD+ depletes rapidly. Glycerate does not give a net production of ATP and also NAD+ is not reduced to NADH and hence NADH is not regenerated. 7i. Lipophilic weak acids uncouple e- transport from ATP synthesis. This shows that proton gradient is a necessity for ATP synthesis and if an alternate route is provided for removal of the proton gradient, ATP synthesis stops. ii. Nigericin and Valinomycin together in presence of K+ uncouple e- transport and ATP synthesis signifying the same result as above. iii. If mitochondria is incubated at ph=9 containing 100M KCl with APD and radiolabeled Pi is moved to another medium containing Valinomycin at ph=7, radiolabeled ATP is synthesized. This indicated tha artificially created proton gradient is sufficient to carry out ATP synthesis. Fall 2003
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Reworded Answers for Finals - F all 2000 : 2b. Each...

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